7UQO

Pathogenesis related 10-10 (S)-tetrahydropapaverine complex

Summary for 7UQO

• Entry DOI: 10.2210/pdb7uqo/pdb
• Descriptor: Pathogenesis Related 10-10 protein, (1~{S})-1-[(3,4-dimethoxyphenyl)methyl]-6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline (3 entities in total)
• Functional Keywords: binding protein, alkaloids, opium poppy, biosynthetic protein, (s)-tetrahydropapaverine, tetrahydropapaverine
• Biological source: Papaver somniferum
• Total number of polymer chains: 1
• Total formula weight: 17969.31

Authors

Carr, S.C.,Ng, K.K.S. (deposition date: 2022-04-19, release date: 2023-03-01, Last modification date: 2023-10-25)

Primary citation

Ozber, N.,Carr, S.C.,Morris, J.S.,Liang, S.,Watkins, J.L.,Caldo, K.M.,Hagel, J.M.,Ng, K.K.S.,Facchini, P.J.
Alkaloid binding to opium poppy major latex proteins triggers structural modification and functional aggregation.
Nat Commun, 13:6768-6768, 2022

PubMed Abstract: Opium poppy accumulates copious amounts of several benzylisoquinoline alkaloids including morphine, noscapine, and papaverine, in the specialized cytoplasm of laticifers, which compose an internal secretory system associated with phloem throughout the plant. The contiguous latex includes an abundance of related proteins belonging to the pathogenesis-related (PR)10 family known collectively as major latex proteins (MLPs) and representing at least 35% of the total cellular protein content. Two latex MLP/PR10 proteins, thebaine synthase and neopione isomerase, have recently been shown to catalyze late steps in morphine biosynthesis previously assigned as spontaneous reactions. Using a combination of sucrose density-gradient fractionation-coupled proteomics, differential scanning fluorimetry, isothermal titration calorimetry, and X-ray crystallography, we show that the major latex proteins are a family of alkaloid-binding proteins that display altered conformation in the presence of certain ligands. Addition of MLP/PR10 proteins to yeast strains engineered with morphine biosynthetic genes from the plant significantly enhanced the conversion of salutaridine to morphinan alkaloids.

PubMed: 36351903
DOI: 10.1038/s41467-022-34313-6

Experimental method

X-RAY DIFFRACTION (1.75 Å)