7UPH

Structure of a ribosome with tethered subunits

これはPDB形式変換不可エントリーです。

7UPH の概要

• エントリーDOI: 10.2210/pdb7uph/pdb
• EMDBエントリー: 26666
• 分子名称: 30S ribosomal protein S14, 5S rRNA, 50S ribosomal protein L2, ... (49 entities in total)
• 機能のキーワード: engineered, tethered, synthetic, ribosome
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 49
• 化学式量合計: 2085073.85

構造登録者

Kim, D.S.,Watkins, A.,Bidstrup, E.,Lee, J.,Topkar, V.V.,Kofman, C.,Schwarz, K.J.,Liu, Y.,Pintilie, G.,Roney, E.,Das, R.,Jewett, M.C. (登録日: 2022-04-15, 公開日: 2022-08-17, 最終更新日: 2025-03-19)

主引用文献

Kim, D.S.,Watkins, A.,Bidstrup, E.,Lee, J.,Topkar, V.,Kofman, C.,Schwarz, K.J.,Liu, Y.,Pintilie, G.,Roney, E.,Das, R.,Jewett, M.C.
Three-dimensional structure-guided evolution of a ribosome with tethered subunits.
Nat.Chem.Biol., 18:990-998, 2022

PubMed Abstract: RNA-based macromolecular machines, such as the ribosome, have functional parts reliant on structural interactions spanning sequence-distant regions. These features limit evolutionary exploration of mutant libraries and confound three-dimensional structure-guided design. To address these challenges, we describe Evolink (evolution and linkage), a method that enables high-throughput evolution of sequence-distant regions in large macromolecular machines, and library design guided by computational RNA modeling to enable exploration of structurally stable designs. Using Evolink, we evolved a tethered ribosome with a 58% increased activity in orthogonal protein translation and a 97% improvement in doubling times in SQ171 cells compared to a previously developed tethered ribosome, and reveal new permissible sequences in a pair of ribosomal helices with previously explored biological function. The Evolink approach may enable enhanced engineering of macromolecular machines for new and improved functions for synthetic biology.

PubMed: 35836020
DOI: 10.1038/s41589-022-01064-w

実験手法

ELECTRON MICROSCOPY (4.18 Å)