7UPB

Prefusion-stabilized Nipah virus fusion protein complexed with Fab 1H1

7UPB の概要

• エントリーDOI: 10.2210/pdb7upb/pdb
• EMDBエントリー: 26660
• 分子名称: Fusion glycoprotein F0, Fab 1H1 heavy chain, Fab 1H1 light chain, ... (4 entities in total)
• 機能のキーワード: henipavirus, nipah virus, niv, f, fusion, prefusion, pref, pre-f, neutralizing antibody, fab, viral protein, viral protein-immune system complex, viral protein/immune system
• 由来する生物種: Nipah henipavirus; 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 232148.96

構造登録者

Byrne, P.O.,McLellan, J.S. (登録日: 2022-04-14, 公開日: 2023-03-29, 最終更新日: 2025-05-28)

主引用文献

Byrne, P.O.,Fisher, B.E.,Ambrozak, D.R.,Blade, E.G.,Tsybovsky, Y.,Graham, B.S.,McLellan, J.S.,Loomis, R.J.
Structural basis for antibody recognition of vulnerable epitopes on Nipah virus F protein.
Nat Commun, 14:1494-1494, 2023

PubMed Abstract: Nipah virus (NiV) is a pathogenic paramyxovirus that causes fatal encephalitis in humans. Two envelope glycoproteins, the attachment protein (G/RBP) and fusion protein (F), facilitate entry into host cells. Due to its vital role, NiV F presents an attractive target for developing vaccines and therapeutics. Several neutralization-sensitive epitopes on the NiV F apex have been described, however the antigenicity of most of the F protein's surface remains uncharacterized. Here, we immunize mice with prefusion-stabilized NiV F and isolate ten monoclonal antibodies that neutralize pseudotyped virus. Cryo-electron microscopy reveals eight neutralization-sensitive epitopes on NiV F, four of which have not previously been described. Novel sites span the lateral and basal faces of NiV F, expanding the known library of vulnerable epitopes. Seven of ten antibodies bind the Hendra virus (HeV) F protein. Multiple sequence alignment suggests that some of these newly identified neutralizing antibodies may also bind F proteins across the Henipavirus genus. This work identifies new epitopes as targets for therapeutics, provides a molecular basis for NiV neutralization, and lays a foundation for development of new cross-reactive antibodies targeting Henipavirus F proteins.

PubMed: 36932063
DOI: 10.1038/s41467-023-36995-y

実験手法

ELECTRON MICROSCOPY (3 Å)