7UO2

E.coli RNaseP Holoenzyme with Mg2+

7UO2 の概要

• エントリーDOI: 10.2210/pdb7uo2/pdb
• EMDBエントリー: 26638
• 分子名称: Ribonuclease P protein component, RNase P RNA, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: ribozyme, protein-rna complex, divalent ion, rna, hydrolase-rna complex, hydrolase/rna
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 135999.59

構造登録者

Huang, W.,Taylor, D.J. (登録日: 2022-04-12, 公開日: 2022-09-28, 最終更新日: 2025-05-28)

主引用文献

Zhu, J.,Huang, W.,Zhao, J.,Huynh, L.,Taylor, D.J.,Harris, M.E.
Structural and mechanistic basis for recognition of alternative tRNA precursor substrates by bacterial ribonuclease P.
Nat Commun, 13:5120-5120, 2022

PubMed Abstract: Binding of precursor tRNAs (ptRNAs) by bacterial ribonuclease P (RNase P) involves an encounter complex (ES) that isomerizes to a catalytic conformation (ES*). However, the structures of intermediates and the conformational changes that occur during binding are poorly understood. Here, we show that pairing between the 5' leader and 3'RCCA extending the acceptor stem of ptRNA inhibits ES* formation. Cryo-electron microscopy single particle analysis reveals a dynamic enzyme that becomes ordered upon formation of ES* in which extended acceptor stem pairing is unwound. Comparisons of structures with alternative ptRNAs reveals that once unwinding is completed RNase P primarily uses stacking interactions and shape complementarity to accommodate alternative sequences at its cleavage site. Our study reveals active site interactions and conformational changes that drive molecular recognition by RNase P and lays the foundation for understanding how binding interactions are linked to helix unwinding and catalysis.

PubMed: 36045135
DOI: 10.1038/s41467-022-32843-7

実験手法

ELECTRON MICROSCOPY (3.1 Å)