7UNJ

De novo designed chlorophyll dimer protein with Zn pheophorbide a methyl ester matching geometry of purple bacterial special pair, SP1-ZnPPaM

7UNJ の概要

• エントリーDOI: 10.2210/pdb7unj/pdb
• 分子名称: SP1-ZnPPaM designed chlorophyll dimer protein, [methyl 9-ethenyl-14-ethyl-3-(3-methoxy-3-oxopropyl)-4,8,13,18-tetramethyl-20-oxophorbine-21-carboxylatato(2-)-kappa~4~N~23~,N~24~,N~25~,N~26~]zinc, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: design, homodimer, rosetta, symmetric, designed, circular tandem repeat protein, ctrp, apo, chlorophyll, de novo protein
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 51285.43

構造登録者

Kennedy, M.A.,Stoddard, B.L.,Ennist, N.M. (登録日: 2022-04-11, 公開日: 2023-04-19, 最終更新日: 2024-06-12)

主引用文献

Ennist, N.M.,Wang, S.,Kennedy, M.A.,Curti, M.,Sutherland, G.A.,Vasilev, C.,Redler, R.L.,Maffeis, V.,Shareef, S.,Sica, A.V.,Hua, A.S.,Deshmukh, A.P.,Moyer, A.P.,Hicks, D.R.,Swartz, A.Z.,Cacho, R.A.,Novy, N.,Bera, A.K.,Kang, A.,Sankaran, B.,Johnson, M.P.,Phadkule, A.,Reppert, M.,Ekiert, D.,Bhabha, G.,Stewart, L.,Caram, J.R.,Stoddard, B.L.,Romero, E.,Hunter, C.N.,Baker, D.
De novo design of proteins housing excitonically coupled chlorophyll special pairs.
Nat.Chem.Biol., 2024

PubMed Abstract: Natural photosystems couple light harvesting to charge separation using a 'special pair' of chlorophyll molecules that accepts excitation energy from the antenna and initiates an electron-transfer cascade. To investigate the photophysics of special pairs independently of the complexities of native photosynthetic proteins, and as a first step toward creating synthetic photosystems for new energy conversion technologies, we designed C-symmetric proteins that hold two chlorophyll molecules in closely juxtaposed arrangements. X-ray crystallography confirmed that one designed protein binds two chlorophylls in the same orientation as native special pairs, whereas a second designed protein positions them in a previously unseen geometry. Spectroscopy revealed that the chlorophylls are excitonically coupled, and fluorescence lifetime imaging demonstrated energy transfer. The cryo-electron microscopy structure of a designed 24-chlorophyll octahedral nanocage with a special pair on each edge closely matched the design model. The results suggest that the de novo design of artificial photosynthetic systems is within reach of current computational methods.

PubMed: 38831036
DOI: 10.1038/s41589-024-01626-0

実験手法

X-RAY DIFFRACTION (2 Å)