7UMD

DENV1 SLA three-way junction RNA (DenvSLAsh)

7UMD の概要

• エントリーDOI: 10.2210/pdb7umd/pdb
• NMR情報: BMRB: 31009
• 分子名称: RNA (40-MER) (1 entity in total)
• 機能のキーワード: flavivirus, 5' utr, sla, viral replication, ns5 promoter, rna, structural genomics, seattle structural genomics center for infectious disease, ssgcid
• 由来する生物種: Dengue virus 1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12929.75

構造登録者

Sun, Y.T.,Varani, G.,Seattle Structural Genomics Center for Infectious Disease (SSGCID) (登録日: 2022-04-06, 公開日: 2022-07-06, 最終更新日: 2024-05-15)

主引用文献

Sun, Y.T.,Varani, G.
Structure of the dengue virus RNA promoter.
Rna, 28:1210-1223, 2022

PubMed Abstract: Dengue virus, a single-stranded positive sense RNA virus, is the most prevalent mosquito-borne pathogen in the world. Like all RNA viruses, it uses conserved structural elements within its genome to control essential replicative steps. A 70 nt stem-loop RNA structure (called SLA), found at the 5'-end of the genome of all flaviviruses, functions as the promoter for viral replication. This highly conserved structure interacts with the viral polymerase NS5 to initiate RNA synthesis. Here, we report the NMR structure of a monomeric SLA from dengue virus serotype 1, assembled to high-resolution from independently folded structural elements. The DENV1 SLA has an L-shaped structure, where the top and side helices are coaxially stacked, and the bottom helix is roughly perpendicular to them. Because the sequence is highly conserved among different flavivirus genomes, it is very likely that the three-dimensional fold and local structure of SLA are also conserved among flaviviruses and required for efficient replication. This work provides structural insight into the dengue promoter and provides the foundation for the discovery of new antiviral drugs that target this essential replicative step.

PubMed: 35750488
DOI: 10.1261/rna.079197.122

実験手法

SOLUTION NMR