7UIN

CryoEM Structure of an Group II Intron Retroelement

7UIN の概要

• エントリーDOI: 10.2210/pdb7uin/pdb
• EMDBエントリー: 26550
• 分子名称: E.r IIC Intron, Group II intron reverse transcriptase/maturase, DNA (37-MER), ... (6 entities in total)
• 機能のキーワード: rna, intron, group ii, maturase, splicing, retrotransposition, rna binding protein-rna-dna complex, rna binding protein/rna/dna
• 由来する生物種: [Eubacterium] rectale; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 267983.70

構造登録者

Chung, K.,Xu, L. (登録日: 2022-03-29, 公開日: 2022-11-23, 最終更新日: 2024-06-12)

主引用文献

Chung, K.,Xu, L.,Chai, P.,Peng, J.,Devarkar, S.C.,Pyle, A.M.
Structures of a mobile intron retroelement poised to attack its structured DNA target
Science, 378:627-634, 2022

PubMed Abstract: Group II introns are ribozymes that catalyze their self-excision and function as retroelements that invade DNA. As retrotransposons, group II introns form ribonucleoprotein (RNP) complexes that roam the genome, integrating by reversal of forward splicing. Here we show that retrotransposition is achieved by a tertiary complex between a structurally elaborate ribozyme, its protein mobility factor, and a structured DNA substrate. We solved cryo-electron microscopy structures of an intact group IIC intron-maturase retroelement that was poised for integration into a DNA stem-loop motif. By visualizing the RNP before and after DNA targeting, we show that it is primed for attack and fits perfectly with its DNA target. This study reveals design principles of a prototypical retroelement and reinforces the hypothesis that group II introns are ancient elements of genetic diversification.

PubMed: 36356138
DOI: 10.1126/science.abq2844

実験手法

ELECTRON MICROSCOPY (2.8 Å)