7UIC

Mediator-PIC Early (Tail A)

7UIC の概要

• エントリーDOI: 10.2210/pdb7uic/pdb
• EMDBエントリー: 26543
• 分子名称: Mediator of RNA polymerase II transcription subunit 2, Mediator of RNA polymerase II transcription subunit 3, Mediator of RNA polymerase II transcription subunit 5, ... (6 entities in total)
• 機能のキーワード: divergent transcription, mediator, rna polymerase ii, pic, pre-initiation, activation, transcription factor, gal4, gal4vp16, transcription
• 由来する生物種: Saccharomyces cerevisiae S288C; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 575145.42

構造登録者

Gorbea Colon, J.J.,Chen, S.-F.,Tsai, K.L.,Murakami, K. (登録日: 2022-03-29, 公開日: 2023-02-15, 最終更新日: 2024-06-12)

主引用文献

Gorbea Colon, J.J.,Palao 3rd, L.,Chen, S.F.,Kim, H.J.,Snyder, L.,Chang, Y.W.,Tsai, K.L.,Murakami, K.
Structural basis of a transcription pre-initiation complex on a divergent promoter.
Mol.Cell, 83:574-, 2023

PubMed Abstract: Most eukaryotic promoter regions are divergently transcribed. As the RNA polymerase II pre-initiation complex (PIC) is intrinsically asymmetric and responsible for transcription in a single direction, it is unknown how divergent transcription arises. Here, the Saccharomyces cerevisiae Mediator complexed with a PIC (Med-PIC) was assembled on a divergent promoter and analyzed by cryoelectron microscopy. The structure reveals two distinct Med-PICs forming a dimer through the Mediator tail module, induced by a homodimeric activator protein localized near the dimerization interface. The tail dimer is associated with ∼80-bp upstream DNA, such that two flanking core promoter regions are positioned and oriented in a suitable form for PIC assembly in opposite directions. Also, cryoelectron tomography visualized the progress of the PIC assembly on the two core promoter regions, providing direct evidence for the role of the Med-PIC dimer in divergent transcription.

PubMed: 36731470
DOI: 10.1016/j.molcel.2023.01.011

実験手法

ELECTRON MICROSCOPY (3.7 Å)