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7UGX

Asp-bound GltPh RSMR mutant in IFS-B1 state

Summary for 7UGX
Entry DOI10.2210/pdb7ugx/pdb
EMDB information26498
DescriptorGlutamate transporter homolog, ASPARTIC ACID, SODIUM ION (3 entities in total)
Functional Keywordsgltph, glutamate transporter, substrate, transport protein
Biological sourcePyrococcus horikoshii
Total number of polymer chains1
Total formula weight44335.30
Authors
Huang, Y.,Boudker, O. (deposition date: 2022-03-25, release date: 2023-03-29, Last modification date: 2023-05-10)
Primary citationHuang, Y.,Reddy, K.D.,Bracken, C.,Qiu, B.,Zhan, W.,Eliezer, D.,Boudker, O.
Environmentally Ultrasensitive Fluorine Probe to Resolve Protein Conformational Ensembles by 19 F NMR and Cryo-EM.
J.Am.Chem.Soc., 145:8583-8592, 2023
Cited by
PubMed Abstract: Limited chemical shift dispersion represents a significant barrier to studying multistate equilibria of large membrane proteins by F NMR. We describe a novel monofluoroethyl F probe that dramatically increases the chemical shift dispersion. The improved conformational sensitivity and line shape enable the detection of previously unresolved states in one-dimensional (1D) F NMR spectra of a 134 kDa membrane transporter. Changes in the populations of these states in response to ligand binding, mutations, and temperature correlate with population changes of distinct conformations in structural ensembles determined by single-particle cryo-electron microscopy (cryo-EM). Thus, F NMR can guide sample preparation to discover and visualize novel conformational states and facilitate image analysis and three-dimensional (3D) classification.
PubMed: 37023263
DOI: 10.1021/jacs.3c01003
PDB entries with the same primary citation
Experimental method
ELECTRON MICROSCOPY (2.96 Å)
Structure validation

229380

건을2024-12-25부터공개중

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