7UGX

Asp-bound GltPh RSMR mutant in IFS-B1 state

7UGX の概要

• エントリーDOI: 10.2210/pdb7ugx/pdb
• EMDBエントリー: 26498
• 分子名称: Glutamate transporter homolog, ASPARTIC ACID, SODIUM ION (3 entities in total)
• 機能のキーワード: gltph, glutamate transporter, substrate, transport protein
• 由来する生物種: Pyrococcus horikoshii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 44335.30

構造登録者

Huang, Y.,Boudker, O. (登録日: 2022-03-25, 公開日: 2023-03-29, 最終更新日: 2023-05-10)

主引用文献

Huang, Y.,Reddy, K.D.,Bracken, C.,Qiu, B.,Zhan, W.,Eliezer, D.,Boudker, O.
Environmentally Ultrasensitive Fluorine Probe to Resolve Protein Conformational Ensembles by 19 F NMR and Cryo-EM.
J.Am.Chem.Soc., 145:8583-8592, 2023

PubMed Abstract: Limited chemical shift dispersion represents a significant barrier to studying multistate equilibria of large membrane proteins by F NMR. We describe a novel monofluoroethyl F probe that dramatically increases the chemical shift dispersion. The improved conformational sensitivity and line shape enable the detection of previously unresolved states in one-dimensional (1D) F NMR spectra of a 134 kDa membrane transporter. Changes in the populations of these states in response to ligand binding, mutations, and temperature correlate with population changes of distinct conformations in structural ensembles determined by single-particle cryo-electron microscopy (cryo-EM). Thus, F NMR can guide sample preparation to discover and visualize novel conformational states and facilitate image analysis and three-dimensional (3D) classification.

PubMed: 37023263
DOI: 10.1021/jacs.3c01003

実験手法

ELECTRON MICROSCOPY (2.96 Å)