7UFL

Crystal structure of chimeric omicron RBD (strain BA.2) complexed with chimeric mouse ACE2

7UFL の概要

• エントリーDOI: 10.2210/pdb7ufl/pdb
• 分子名称: Angiotensin-converting enzyme 2, Spike protein S1, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (9 entities in total)
• 機能のキーワード: rbd, ace2, viral protein-hydrolase complex, viral protein/hydrolase
• 由来する生物種: Mus musculus (Mouse); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 190432.24

構造登録者

Zhang, W.,Shi, K.,Geng, Q.,Ye, G.,Aihara, H.,Li, F. (登録日: 2022-03-22, 公開日: 2022-10-19, 最終更新日: 2024-10-23)

主引用文献

Zhang, W.,Shi, K.,Geng, Q.,Ye, G.,Aihara, H.,Li, F.
Structural basis for mouse receptor recognition by SARS-CoV-2 omicron variant.
Proc.Natl.Acad.Sci.USA, 119:e2206509119-e2206509119, 2022

PubMed Abstract: The sudden emergence and rapid spread of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) omicron variant has raised questions about its animal reservoir. Here, we investigated receptor recognition of the omicron's receptor-binding domain (RBD), focusing on four of its mutations (Q493R, Q498R, N501Y, and Y505H) surrounding two mutational hotspots. These mutations have variable effects on the RBD's affinity for human angiotensin-converting enzyme 2 (ACE2), but they all enhance the RBD's affinity for mouse ACE2. We further determined the crystal structure of omicron RBD complexed with mouse ACE2. The structure showed that all four mutations are viral adaptations to mouse ACE2: three of them (Q493R, Q498R, and Y505H) are uniquely adapted to mouse ACE2, whereas the other one (N501Y) is adapted to both human ACE2 and mouse ACE2. These data reveal that the omicron RBD was well adapted to mouse ACE2 before omicron started to infect humans, providing insight into the potential evolutionary origin of the omicron variant.

PubMed: 36256797
DOI: 10.1073/pnas.2206509119

実験手法

X-RAY DIFFRACTION (2.84 Å)