7U5J

Cryo-EM Structure of ALDOA

7U5J の概要

• エントリーDOI: 10.2210/pdb7u5j/pdb
• EMDBエントリー: 26352
• 分子名称: Fructose-bisphosphate aldolase (2 entities in total)
• 機能のキーワード: aldoa, fructose-bisphosphate aldolase, aldolase, lyase
• 由来する生物種: Bos taurus (cattle)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 157952.42

構造登録者

Morgan, C.E.,Zhang, Z.,Yu, E.W. (登録日: 2022-03-02, 公開日: 2022-12-14, 最終更新日: 2024-06-12)

主引用文献

Morgan, C.E.,Zhang, Z.,Miyagi, M.,Golczak, M.,Yu, E.W.
Toward structural-omics of the bovine retinal pigment epithelium.
Cell Rep, 41:111876-111876, 2022

PubMed Abstract: The use of an integrated systems biology approach to investigate tissues and organs has been thought to be impracticable in the field of structural biology, where the techniques mainly focus on determining the structure of a particular biomacromolecule of interest. Here, we report the use of cryoelectron microscopy (cryo-EM) to define the composition of a raw bovine retinal pigment epithelium (RPE) lysate. From this sample, we simultaneously identify and solve cryo-EM structures of seven different RPE enzymes whose functions affect neurotransmitter recycling, iron metabolism, gluconeogenesis, glycolysis, axonal development, and energy homeostasis. Interestingly, dysfunction of these important proteins has been directly linked to several neurodegenerative disorders, including Huntington's disease, amyotrophic lateral sclerosis (ALS), Parkinson's disease, Alzheimer's disease, and schizophrenia. Our work underscores the importance of cryo-EM in facilitating tissue and organ proteomics at the atomic level.

PubMed: 36577381
DOI: 10.1016/j.celrep.2022.111876

実験手法

ELECTRON MICROSCOPY (2.96 Å)