7TXM

Oxidized Structure of RexT

7TXM の概要

• エントリーDOI: 10.2210/pdb7txm/pdb
• 分子名称: Transcriptional regulator, HYDROGEN PEROXIDE (3 entities in total)
• 機能のキーワード: redox sensing transcriptional regulator, dna binding protein
• 由来する生物種: Nostoc sp. PCC 7120 = FACHB-418
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 25635.76

構造登録者

Bridwell-Rabb, J.,Li, B. (登録日: 2022-02-09, 公開日: 2022-03-23, 最終更新日: 2024-10-30)

主引用文献

Li, B.,Jo, M.,Liu, J.,Tian, J.,Canfield, R.,Bridwell-Rabb, J.
Structural and mechanistic basis for redox sensing by the cyanobacterial transcription regulator RexT.
Commun Biol, 5:275-275, 2022

PubMed Abstract: Organisms have a myriad of strategies for sensing, responding to, and combating reactive oxygen species, which are unavoidable consequences of aerobic life. In the heterocystous cyanobacterium Nostoc sp. PCC 7120, one such strategy is the use of an ArsR-SmtB transcriptional regulator RexT that senses HO and upregulates expression of thioredoxin to maintain cellular redox homeostasis. Different from many other members of the ArsR-SmtB family which bind metal ions, RexT has been proposed to use disulfide bond formation as a trigger to bind and release DNA. Here, we present high-resolution crystal structures of RexT in the reduced and HO-treated states. These structures reveal that RexT showcases the ArsR-SmtB winged-helix-turn-helix fold and forms a vicinal disulfide bond to orchestrate a response to HO. The importance of the disulfide-forming Cys residues was corroborated using site-directed mutagenesis, mass spectrometry, and HO-consumption assays. Furthermore, an entrance channel for HO was identified and key residues implicated in HO activation were pinpointed. Finally, bioinformatics analysis of the ArsR-SmtB family indicates that the vicinal disulfide "redox switch" is a unique feature of cyanobacteria in the Nostocales order, presenting an interesting case where an ArsR-SmtB protein scaffold has been evolved to showcase peroxidatic activity and facilitate redox-based regulation.

PubMed: 35347217
DOI: 10.1038/s42003-022-03226-x

実験手法

X-RAY DIFFRACTION (2.16 Å)