7TXI

Cryo-EM of A. pernix flagellum

7TXI の概要

• エントリーDOI: 10.2210/pdb7txi/pdb
• EMDBエントリー: 26158
• 分子名称: Probable flagellin 1 (1 entity in total)
• 機能のキーワード: helical symmetry, flagellum, cell appendage, structural protein
• 由来する生物種: Aeropyrum pernix
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21371.59

構造登録者

Wang, F.,Cvirkaite-Krupovic, V.,Baquero, D.P.,Krupovic, M.,Egelman, E.H. (登録日: 2022-02-09, 公開日: 2022-06-01, 最終更新日: 2025-05-28)

主引用文献

Kreutzberger, M.A.B.,Cvirkaite-Krupovic, V.,Liu, Y.,Baquero, D.P.,Liu, J.,Sonani, R.R.,Calladine, C.R.,Wang, F.,Krupovic, M.,Egelman, E.H.
The evolution of archaeal flagellar filaments.
Proc.Natl.Acad.Sci.USA, 120:e2304256120-e2304256120, 2023

PubMed Abstract: Flagellar motility has independently arisen three times during evolution: in bacteria, archaea, and eukaryotes. In prokaryotes, the supercoiled flagellar filaments are composed largely of a single protein, bacterial or archaeal flagellin, although these two proteins are not homologous, while in eukaryotes, the flagellum contains hundreds of proteins. Archaeal flagellin and archaeal type IV pilin are homologous, but how archaeal flagellar filaments (AFFs) and archaeal type IV pili (AT4Ps) diverged is not understood, in part, due to the paucity of structures for AFFs and AT4Ps. Despite having similar structures, AFFs supercoil, while AT4Ps do not, and supercoiling is essential for the function of AFFs. We used cryo-electron microscopy to determine the atomic structure of two additional AT4Ps and reanalyzed previous structures. We find that all AFFs have a prominent 10-strand packing, while AT4Ps show a striking structural diversity in their subunit packing. A clear distinction between all AFF and all AT4P structures involves the extension of the N-terminal α-helix with polar residues in the AFFs. Additionally, we characterize a flagellar-like AT4P from with filament and subunit structure similar to that of AFFs which can be viewed as an evolutionary link, showing how the structural diversity of AT4Ps likely allowed for an AT4P to evolve into a supercoiling AFF.

PubMed: 37399404
DOI: 10.1073/pnas.2304256120

実験手法

ELECTRON MICROSCOPY (3.5 Å)