7TUL

Woodchuck hepatitis small surface protein without cytosolic and antigenic loops

7TUL の概要

• エントリーDOI: 10.2210/pdb7tul/pdb
• EMDBエントリー: 26118
• 分子名称: Large envelope protein (1 entity in total)
• 機能のキーワード: whv, svp, whsag, envelope protein, viral protein
• 由来する生物種: Woodchuck hepatitis virus (WHV)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 51301.27

構造登録者

Liu, H.,Wang, J.C.Y. (登録日: 2022-02-02, 公開日: 2022-08-24, 最終更新日: 2024-02-21)

主引用文献

Liu, H.,Hong, X.,Xi, J.,Menne, S.,Hu, J.,Wang, J.C.
Cryo-EM structures of human hepatitis B and woodchuck hepatitis virus small spherical subviral particles.
Sci Adv, 8:eabo4184-eabo4184, 2022

PubMed Abstract: The loss of detectable hepatitis B surface antigen (HBsAg) is considered a functional cure in chronic hepatitis B. Naturally, HBsAg can be incorporated into the virion envelope or assembled into subviral particles (SVPs) with lipid from host cells. Until now, there has been no detailed structure of HBsAg, and the published SVP structures are controversial. Here, we report the first subnanometer-resolution structures of spherical SVP from hepatitis B virus (HBV) and the related woodchuck hepatitis virus (WHV) determined by cryo-electron microscopy in combination with AlphaFold2 prediction. Both structures showed unique rhombicuboctahedral symmetry with 24 protruding spikes comprising dimer of small HBsAg with four helical domains. The lipid moiety in the SVP is organized in a noncanonical lipid patch instead of a lipid bilayer, which can accommodate the exposed hydrophobic surface and modulate particle stability. Together, these findings advance our knowledge of viral membrane organization and the structures of HBV and WHV spherical SVPs.

PubMed: 35930632
DOI: 10.1126/sciadv.abo4184

実験手法

ELECTRON MICROSCOPY (6.5 Å)