7TQF

Selenium-incorporated nitrogenase Fe protein (Av2-Se) from A. vinelandii (22 mM KSeCN)

7TQF の概要

• エントリーDOI: 10.2210/pdb7tqf/pdb
• 分子名称: Nitrogenase iron protein 1, IRON/SULFUR CLUSTER, Fe4-Se4 cluster, ... (6 entities in total)
• 機能のキーワード: nitrogenase, metalloprotein, iron sulfur cluster, oxidoreductase
• 由来する生物種: Azotobacter vinelandii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32890.61

構造登録者

Buscagan, T.M.,Kaiser, J.T.,Rees, D.C. (登録日: 2022-01-26, 公開日: 2022-09-14, 最終更新日: 2023-10-18)

主引用文献

Buscagan, T.M.,Kaiser, J.T.,Rees, D.C.
Selenocyanate derived Se-incorporation into the Nitrogenase Fe protein cluster.
Elife, 11:-, 2022

PubMed Abstract: The nitrogenase Fe protein mediates ATP-dependent electron transfer to the nitrogenase MoFe protein during nitrogen fixation, in addition to catalyzing MoFe protein-independent substrate (CO) reduction and facilitating MoFe protein metallocluster biosynthesis. The precise role(s) of the Fe protein FeS cluster in some of these processes remains ill-defined. Herein, we report crystallographic data demonstrating ATP-dependent chalcogenide exchange at the FeS cluster of the nitrogenase Fe protein when potassium selenocyanate is used as the selenium source, an unexpected result as the Fe protein cluster is not traditionally perceived as a site of substrate binding within nitrogenase. The observed chalcogenide exchange illustrates that this FeS cluster is capable of core substitution reactions under certain conditions, adding to the Fe protein's repertoire of unique properties.

PubMed: 35904245
DOI: 10.7554/eLife.79311

実験手法

X-RAY DIFFRACTION (1.45 Å)