7THR

Cryo-electron microscopy of Adeno-associated virus serotype 4 at 2.2 A

これはPDB形式変換不可エントリーです。

7THR の概要

• エントリーDOI: 10.2210/pdb7thr/pdb
• EMDBエントリー: 25903
• 分子名称: Capsid, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: aav4, adeno-associated virus, serotype 4, virus like particle
• 由来する生物種: Adeno-associated virus - 4
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 80736.63

構造登録者

Zane, G.M.,Silveria, M.A.,Meyer, N.L.,White, T.A.,Chapman, M.S. (登録日: 2022-01-11, 公開日: 2023-01-25, 最終更新日: 2024-06-05)

主引用文献

Zane, G.,Silveria, M.,Meyer, N.,White, T.,Duan, R.,Zou, X.,Chapman, M.
Cryo-EM structure of adeno-associated virus 4 at 2.2 angstrom resolution.
Acta Crystallogr D Struct Biol, 79:140-153, 2023

PubMed Abstract: Adeno-associated virus (AAV) is the vector of choice for several approved gene-therapy treatments and is the basis for many ongoing clinical trials. Various strains of AAV exist (referred to as serotypes), each with their own transfection characteristics. Here, a high-resolution cryo-electron microscopy structure (2.2 Å) of AAV serotype 4 (AAV4) is presented. The receptor responsible for transduction of the AAV4 clade of AAV viruses (including AAV11, AAV12 and AAVrh32.33) is unknown. Other AAVs interact with the same cell receptor, adeno-associated virus receptor (AAVR), in one of two different ways. AAV5-like viruses interact exclusively with the polycystic kidney disease-like 1 (PKD1) domain of AAVR, while most other AAVs interact primarily with the PKD2 domain. A comparison of the present AAV4 structure with prior corresponding structures of AAV5, AAV2 and AAV1 in complex with AAVR provides a foundation for understanding why the AAV4-like clade is unable to interact with either PKD1 or PKD2 of AAVR. The conformation of the AAV4 capsid in variable regions I, III, IV and V on the viral surface appears to be sufficiently different from AAV2 to ablate binding with PKD2. Differences between AAV4 and AAV5 in variable region VII appear to be sufficient to exclude binding with PKD1.

PubMed: 36762860
DOI: 10.1107/S2059798322012190

実験手法

ELECTRON MICROSCOPY (2.21 Å)