7TH0

Escherichia coli RpnA-S

7TH0 の概要

• エントリーDOI: 10.2210/pdb7th0/pdb
• 分子名称: Recombination-promoting nuclease RpnA (2 entities in total)
• 機能のキーワード: toxin-antitoxin phage defense endonuclease abortive infection, antitoxin
• 由来する生物種: Escherichia coli str. K-12 substr. MG1655
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5517.86

構造登録者

Zhong, A.,Hickman, A.B.,Storz, G.,Dyda, F. (登録日: 2022-01-10, 公開日: 2023-03-29, 最終更新日: 2024-11-13)

主引用文献

Zhong, A.,Jiang, X.,Hickman, A.B.,Klier, K.,Teodoro, G.I.C.,Dyda, F.,Laub, M.T.,Storz, G.
Toxic antiphage defense proteins inhibited by intragenic antitoxin proteins.
Proc.Natl.Acad.Sci.USA, 120:e2307382120-e2307382120, 2023

PubMed Abstract: Recombination-promoting nuclease (Rpn) proteins are broadly distributed across bacterial phyla, yet their functions remain unclear. Here, we report that these proteins are toxin-antitoxin systems, comprised of genes-within-genes, that combat phage infection. We show the small, highly variable Rpn -terminal domains (Rpn), which are translated separately from the full-length proteins (Rpn), directly block the activities of the toxic Rpn. The crystal structure of RpnA revealed a dimerization interface encompassing α helix that can have four amino acid repeats whose number varies widely among strains of the same species. Consistent with strong selection for the variation, we document that plasmid-encoded RpnP2 protects against certain phages. We propose that many more intragenic-encoded proteins that serve regulatory roles remain to be discovered in all organisms.

PubMed: 37487082
DOI: 10.1073/pnas.2307382120

実験手法

X-RAY DIFFRACTION (1.9 Å)