7TGG

Cryo-EM structure of PilA-N and PilA-C from Geobacter sulfurreducens

7TGG の概要

• エントリーDOI: 10.2210/pdb7tgg/pdb
• EMDBエントリー: 25881
• 分子名称: Geopilin domain 1 protein, Geopilin domain 2 protein (2 entities in total)
• 機能のキーワード: helical symmetry, filament, pili, type iv pili, pseudo pili, protein fibril
• 由来する生物種: Geobacter sulfurreducens; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 23094.13

構造登録者

Wang, F.,Mustafa, K.,Chan, C.H.,Joshi, K.,Bond, D.R.,Hochbaum, A.I.,Egelman, E.H. (登録日: 2022-01-07, 公開日: 2022-02-23, 最終更新日: 2025-05-14)

主引用文献

Wang, F.,Mustafa, K.,Suciu, V.,Joshi, K.,Chan, C.H.,Choi, S.,Su, Z.,Si, D.,Hochbaum, A.I.,Egelman, E.H.,Bond, D.R.
Cryo-EM structure of an extracellular Geobacter OmcE cytochrome filament reveals tetrahaem packing.
Nat Microbiol, 7:1291-1300, 2022

PubMed Abstract: Electrically conductive appendages from the anaerobic bacterium Geobacter sulfurreducens were first observed two decades ago, with genetic and biochemical data suggesting that conductive fibres were type IV pili. Recently, an extracellular conductive filament of G. sulfurreducens was found to contain polymerized c-type cytochrome OmcS subunits, not pilin subunits. Here we report that G. sulfurreducens also produces a second, thinner appendage comprised of cytochrome OmcE subunits and solve its structure using cryo-electron microscopy at ~4.3 Å resolution. Although OmcE and OmcS subunits have no overall sequence or structural similarities, upon polymerization both form filaments that share a conserved haem packing arrangement in which haems are coordinated by histidines in adjacent subunits. Unlike OmcS filaments, OmcE filaments are highly glycosylated. In extracellular fractions from G. sulfurreducens, we detected type IV pili comprising PilA-N and -C chains, along with abundant B-DNA. OmcE is the second cytochrome filament to be characterized using structural and biophysical methods. We propose that there is a broad class of conductive bacterial appendages with conserved haem packing (rather than sequence homology) that enable long-distance electron transport to chemicals or other microbial cells.

PubMed: 35798889
DOI: 10.1038/s41564-022-01159-z

実験手法

ELECTRON MICROSCOPY (4.1 Å)