7TE2

Crystal Structure of AerR from Rhodobacter capsulatus at 2.25 A.

7TE2 の概要

• エントリーDOI: 10.2210/pdb7te2/pdb
• 分子名称: AerR, COBALAMIN, HEXANE-1,6-DIOL, ... (5 entities in total)
• 機能のキーワード: transcription, b12 binding, transcription regulator, photosynthesis, gene regulator
• 由来する生物種: Rhodobacter capsulatus Y262
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 24309.34

構造登録者

Dragnea, V.,Gonzalez-Gutierrez, G.,Bauer, C.E. (登録日: 2022-01-04, 公開日: 2022-08-31, 最終更新日: 2024-02-28)

主引用文献

Dragnea, V.,Gonzalez-Gutierrez, G.,Bauer, C.E.
Structural Analyses of CrtJ and Its B 12 -Binding Co-Regulators SAerR and LAerR from the Purple Photosynthetic Bacterium Rhodobacter capsulatus.
Microorganisms, 10:-, 2022

PubMed Abstract: Among purple photosynthetic bacteria, the transcription factor CrtJ is a major regulator of photosystem gene expression. Depending on growing conditions, CrtJ can function as an aerobic repressor or an anaerobic activator of photosystem genes. Recently, CrtJ's activity was shown to be modulated by two size variants of a B binding co-regulator called SAerR and LAerR in . The short form, SAerR, promotes CrtJ repression, while the longer variant, LAerR, converts CrtJ into an activator. In this study, we solved the crystal structure of SAerR at a 2.25 Å resolution. Hydroxycobalamin bound to SAerR is sandwiched between a 4-helix bundle cap, and a Rossman fold. This structure is similar to a AerR-like domain present in CarH from , which is a combined photoreceptor/transcription regulator. We also utilized AlphaFold software to predict structures for the LAerR, CrtJ, SAerR-CrtJ and LAerR-CrtJ co-complexes. These structures provide insights into the role of B and an LAerR N-terminal extension in regulating the activity of CrtJ.

PubMed: 35630357
DOI: 10.3390/microorganisms10050912

実験手法

X-RAY DIFFRACTION (2.25 Å)