7TD7

Crystal structure of an E. coli thiM riboswitch bound to thiamine, manganese ions

7TD7 の概要

• エントリーDOI: 10.2210/pdb7td7/pdb
• 分子名称: thiM riboswitch RNA (78-MER), MANGANESE (II) ION, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: rna
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 27167.84

構造登録者

Nuthanakanti, A.,Serganov, A. (登録日: 2021-12-30, 公開日: 2022-02-16, 最終更新日: 2023-10-18)

主引用文献

Zeller, M.J.,Nuthanakanti, A.,Li, K.,Aube, J.,Serganov, A.,Weeks, K.M.
Subsite Ligand Recognition and Cooperativity in the TPP Riboswitch: Implications for Fragment-Linking in RNA Ligand Discovery.
Acs Chem.Biol., 17:438-448, 2022

PubMed Abstract: RNA molecules can show high levels of cooperativity in their global folding and interactions with divalent ions. However, cooperativity at individual ligand-RNA interaction sites remains poorly understood. Here, we investigated the binding of thiamine and methylene diphosphonic acid (MDP, a soluble structural analogue of pyrophosphate) to the thiamine pyrophosphate riboswitch. These ligands each bind weakly at proximal subsites, with 10 μM and 1 mM affinities, respectively. The affinity of MDP moderately improves when thiamine or thiamine-like fragments are pre-bound to the RNA. Covalent linking of thiamine and MDP substantially increases riboswitch binding to a notable high affinity of 20 nM. Crystal structures and single-molecule correlated chemical probing revealed favorable induced fit effects upon binding of individual ligands and, unexpectedly, a substantial thermodynamically unfavorable RNA structural rearrangement upon binding of the linked thiamine-MDP ligand. Thus, linking of two ligands of modest affinity, accompanied by an unfavorable structural rearrangement, still yields a potent linked RNA-binding compound. Since complex ligands often bind riboswitches and other RNAs at proximal subsites, principles derived from this work inform and support fragment-linking strategies for identifying small molecules that interact with RNA specifically and with high affinity.

PubMed: 35060698
DOI: 10.1021/acschembio.1c00880

実験手法

X-RAY DIFFRACTION (2.95 Å)