7T1J

Crystal structure of RUBISCO from Rhodospirillaceae bacterium BRH_c57

7T1J の概要

• エントリーDOI: 10.2210/pdb7t1j/pdb
• 分子名称: Ribulose bisphosphate carboxylase, 2-CARBOXYARABINITOL-1,5-DIPHOSPHATE, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: ribulose-1, 5-bisphosphate carboxylase-oxygenase, lyase
• 由来する生物種: Rhodospirillaceae bacterium BRH_c57
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 610372.30

構造登録者

Pereira, J.H.,Liu, A.K.,Shih, P.M.,Adams, P.D. (登録日: 2021-12-02, 公開日: 2022-09-07, 最終更新日: 2023-11-15)

主引用文献

Liu, A.K.,Pereira, J.H.,Kehl, A.J.,Rosenberg, D.J.,Orr, D.J.,Chu, S.K.S.,Banda, D.M.,Hammel, M.,Adams, P.D.,Siegel, J.B.,Shih, P.M.
Structural plasticity enables evolution and innovation of RuBisCO assemblies.
Sci Adv, 8:eadc9440-eadc9440, 2022

PubMed Abstract: Oligomerization is a core structural feature that defines the form and function of many proteins. Most proteins form molecular complexes; however, there remains a dearth of diversity-driven structural studies investigating the evolutionary trajectory of these assemblies. Ribulose-1,5-bisphosphate carboxylase-oxygenase (RuBisCO) is one such enzyme that adopts multiple assemblies, although the origins and distribution of its different oligomeric states remain cryptic. Here, we retrace the evolution of ancestral and extant form II RuBisCOs, revealing a complex and diverse history of oligomerization. We structurally characterize a newly discovered tetrameric RuBisCO, elucidating how solvent-exposed surfaces can readily adopt new interactions to interconvert or give rise to new oligomeric states. We further use these principles to engineer and demonstrate how changes in oligomerization can be mediated by relatively few mutations. Our findings yield insight into how structural plasticity may give rise to new oligomeric states.

PubMed: 36026446
DOI: 10.1126/sciadv.adc9440

実験手法

X-RAY DIFFRACTION (1.96 Å)