7ST9

Open state of Rad24-RFC:9-1-1 bound to a 5' ss/dsDNA junction

7ST9 の概要

• エントリーDOI: 10.2210/pdb7st9/pdb
• EMDBエントリー: 25422
• 分子名称: Checkpoint protein RAD24, DNA (5'-D(P*CP*GP*CP*TP*CP*CP*TP*TP*CP*CP*TP*GP*AP*CP*TP*CP*GP*TP*CP*C)-3'), PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER, ... (16 entities in total)
• 機能のキーワード: dna damage, dna replication, dna sliding clamp, replication, replication-dna complex, replication/dna
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 431630.03

構造登録者

Castaneda, J.C.,Schrecker, M.,Remus, D.,Hite, R.K. (登録日: 2021-11-12, 公開日: 2022-03-23, 最終更新日: 2024-06-05)

主引用文献

Castaneda, J.C.,Schrecker, M.,Remus, D.,Hite, R.K.
Mechanisms of loading and release of the 9-1-1 checkpoint clamp.
Nat.Struct.Mol.Biol., 29:369-375, 2022

PubMed Abstract: Single-stranded or double-stranded DNA junctions with recessed 5' ends serve as loading sites for the checkpoint clamp, 9-1-1, which mediates activation of the apical checkpoint kinase, ATR. However, the basis for 9-1-1's recruitment to 5' junctions is unclear. Here, we present structures of the yeast checkpoint clamp loader, Rad24-replication factor C (RFC), in complex with 9-1-1 and a 5' junction and in a post-ATP-hydrolysis state. Unexpectedly, 9-1-1 adopts both closed and planar open states in the presence of Rad24-RFC and DNA. Moreover, Rad24-RFC associates with the DNA junction in the opposite orientation of processivity clamp loaders with Rad24 exclusively coordinating the double-stranded region. ATP hydrolysis stimulates conformational changes in Rad24-RFC, leading to disengagement of DNA-loaded 9-1-1. Together, these structures explain 9-1-1's recruitment to 5' junctions and reveal new principles of sliding clamp loading.

PubMed: 35314831
DOI: 10.1038/s41594-022-00741-7

実験手法

ELECTRON MICROSCOPY (2.2 Å)