7SRK

Single chain trimer HLA-A*24:02 (Y108C, A163C) with 8mer peptide YPPVPETF

Summary for 7SRK

• Entry DOI: 10.2210/pdb7srk/pdb
• Descriptor: RPA-related protein RADX peptide,Beta-2-microglobulin,MHC class I antigen chimera, VHH (3 entities in total)
• Functional Keywords: hla, vhh, hpv, sct, immune system
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 4
• Total formula weight: 119773.70

Authors

Finton, K.A.K.,Rupert, P.B. (deposition date: 2021-11-08, release date: 2022-11-23, Last modification date: 2023-10-25)

Primary citation

Finton, K.A.K.,Rupert, P.B.,Friend, D.J.,Dinca, A.,Lovelace, E.S.,Buerger, M.,Rusnac, D.V.,Foote-McNabb, U.,Chour, W.,Heath, J.R.,Campbell, J.S.,Pierce, R.H.,Strong, R.K.
Effects of HLA single chain trimer design on peptide presentation and stability.
Front Immunol, 14:1170462-1170462, 2023

PubMed Abstract: MHC class I "" molecules, coupling MHC heavy chain, β-microglobulin, and a specific peptide into a single polypeptide chain, are widely used in research. To more fully understand caveats associated with this design that may affect its use for basic and translational studies, we evaluated a set of engineered single-chain trimers with combinations of stabilizing mutations across eight different classical and non-classical human class I alleles with 44 different peptides, including a novel human/murine chimeric design. While, overall, single-chain trimers accurately recapitulate native molecules, care was needed in selecting designs for studying peptides longer or shorter than 9-mers, as single-chain trimer design could affect peptide conformation. In the process, we observed that of peptide binding were often discordant with and that yields and stabilities varied widely with construct design. We also developed novel reagents to improve the crystallizability of these proteins and confirmed novel modes of peptide presentation.

PubMed: 37207206
DOI: 10.3389/fimmu.2023.1170462

Experimental method

X-RAY DIFFRACTION (2.5 Å)