7SP5

Crystal Structure of a Eukaryotic Phosphate Transporter

「4J05」から置き換えられました

7SP5 の概要

• エントリーDOI: 10.2210/pdb7sp5/pdb
• 分子名称: Phosphate transporter, PHOSPHATE ION, nonyl beta-D-glucopyranoside (3 entities in total)
• 機能のキーワード: major facilitator, phosphate import, phosphate binding, membrane protein, transport protein
• 由来する生物種: Serendipita indica (Root endophyte fungus, Piriformospora indica)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 118799.26

構造登録者

Stroud, R.M.,Pedersen, B.P.,Kumar, H.,Waight, A.B.,Risenmay, A.J.,Roe-Zurz, Z.,Chau, B.H.,Schlessinger, A.,Bonomi, M.,Harries, W.,Sali, A.,Johri, A.K.,Finer-Moore, J. (登録日: 2021-11-02, 公開日: 2021-11-17, 最終更新日: 2024-05-22)

主引用文献

Pedersen, B.P.,Kumar, H.,Waight, A.B.,Risenmay, A.J.,Roe-Zurz, Z.,Chau, B.H.,Schlessinger, A.,Bonomi, M.,Harries, W.,Sali, A.,Johri, A.K.,Stroud, R.M.
Crystal structure of a eukaryotic phosphate transporter.
Nature, 496:533-536, 2013

PubMed Abstract: Phosphate is crucial for structural and metabolic needs, including nucleotide and lipid synthesis, signalling and chemical energy storage. Proton-coupled transporters of the major facilitator superfamily (MFS) are essential for phosphate uptake in plants and fungi, and also have a function in sensing external phosphate levels as transceptors. Here we report the 2.9 Å structure of a fungal (Piriformospora indica) high-affinity phosphate transporter, PiPT, in an inward-facing occluded state, with bound phosphate visible in the membrane-buried binding site. The structure indicates both proton and phosphate exit pathways and suggests a modified asymmetrical 'rocker-switch' mechanism of phosphate transport. PiPT is related to several human transporter families, most notably the organic cation and anion transporters of the solute carrier family (SLC22), which are implicated in cancer-drug resistance. We modelled representative cation and anion SLC22 transporters based on the PiPT structure to surmise the structural basis for substrate binding and charge selectivity in this important family. The PiPT structure demonstrates and expands on principles of substrate transport by the MFS transporters and illuminates principles of phosphate uptake in particular.

PubMed: 23542591
DOI: 10.1038/nature12042

実験手法

X-RAY DIFFRACTION (2.9 Å)