7SLH
Engineered sperm whale myoglobin-based carbene transferase MbBTIC-C3
Summary for 7SLH
| Entry DOI | 10.2210/pdb7slh/pdb |
| Descriptor | Myoglobin, PROTOPORPHYRIN IX CONTAINING FE, SULFATE ION, ... (4 entities in total) |
| Functional Keywords | metalloprotein, myoglobin, heme, transferase, oxygen transport, oxygen storage |
| Biological source | Physeter catodon (Sperm whale) |
| Total number of polymer chains | 1 |
| Total formula weight | 18622.02 |
| Authors | Jenkins, J.L.,Vargas, D.,Ren, X.,Fasan, R. (deposition date: 2021-10-24, release date: 2023-05-03, Last modification date: 2024-05-29) |
| Primary citation | Vargas, D.A.,Ren, X.,Sengupta, A.,Zhu, L.,Roy, S.,Garcia-Borras, M.,Houk, K.N.,Fasan, R. Biocatalytic strategy for the construction of sp 3 -rich polycyclic compounds from directed evolution and computational modelling. Nat.Chem., 16:817-826, 2024 Cited by PubMed Abstract: Catalysis with engineered enzymes has provided more efficient routes for the production of active pharmaceutical agents. However, the potential of biocatalysis to assist in early-stage drug discovery campaigns remains largely untapped. In this study, we have developed a biocatalytic strategy for the construction of sp-rich polycyclic compounds via the intramolecular cyclopropanation of benzothiophenes and related heterocycles. Two carbene transferases with complementary regioisomer selectivity were evolved to catalyse the stereoselective cyclization of benzothiophene substrates bearing diazo ester groups at the C2 or C3 position of the heterocycle. The detailed mechanisms of these reactions were elucidated by a combination of crystallographic and computational analyses. Leveraging these insights, the substrate scope of one of the biocatalysts could be expanded to include previously unreactive substrates, highlighting the value of integrating evolutionary and rational strategies to develop enzymes for new-to-nature transformations. The molecular scaffolds accessed here feature a combination of three-dimensional and stereochemical complexity with 'rule-of-three' properties, which should make them highly valuable for fragment-based drug discovery campaigns. PubMed: 38351380DOI: 10.1038/s41557-023-01435-3 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.15 Å) |
Structure validation
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