7SJ1

Structure of shaker-W434F

7SJ1 の概要

• エントリーDOI: 10.2210/pdb7sj1/pdb
• EMDBエントリー: 25152
• 分子名称: Potassium voltage-gated channel protein Shaker, (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate, POTASSIUM ION, ... (4 entities in total)
• 機能のキーワード: potassium channel, membrane protein
• 由来する生物種: Drosophila melanogaster (Fruit fly)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 302145.75

構造登録者

Tan, X.,Bae, C.,Stix, R.,Fernandez, A.I.,Huffer, K.,Chang, T.,Jiang, J.,Faraldo-Gomez, J.D.,Swartz, K.J. (登録日: 2021-10-15, 公開日: 2022-03-30, 最終更新日: 2024-06-05)

主引用文献

Tan, X.F.,Bae, C.,Stix, R.,Fernandez-Marino, A.I.,Huffer, K.,Chang, T.H.,Jiang, J.,Faraldo-Gomez, J.D.,Swartz, K.J.
Structure of the Shaker Kv channel and mechanism of slow C-type inactivation.
Sci Adv, 8:eabm7814-eabm7814, 2022

PubMed Abstract: Voltage-activated potassium (Kv) channels open upon membrane depolarization and proceed to spontaneously inactivate. Inactivation controls neuronal firing rates and serves as a form of short-term memory and is implicated in various human neurological disorders. Here, we use high-resolution cryo-electron microscopy and computer simulations to determine one of the molecular mechanisms underlying this physiologically crucial process. Structures of the activated Shaker Kv channel and of its W434F mutant in lipid bilayers demonstrate that C-type inactivation entails the dilation of the ion selectivity filter and the repositioning of neighboring residues known to be functionally critical. Microsecond-scale molecular dynamics trajectories confirm that these changes inhibit rapid ion permeation through the channel. This long-sought breakthrough establishes how eukaryotic K channels self-regulate their functional state through the plasticity of their selectivity filters.

PubMed: 35302848
DOI: 10.1126/sciadv.abm7814

実験手法

ELECTRON MICROSCOPY (2.9 Å)