7SFS

In situ cryo-EM structure of bacteriophage Sf6 portal:gp7 complex at 2.7A resolution

7SFS の概要

• エントリーDOI: 10.2210/pdb7sfs/pdb
• EMDBエントリー: 25101
• 分子名称: Gene 3 protein, Gene 7 protein (2 entities in total)
• 機能のキーワード: in situ, phage, portal, gp7, gp3, structural protein
• 由来する生物種: Shigella phage Sf6 (Shigella flexneri bacteriophage VI, Bacteriophage SfVI); 詳細
• タンパク質・核酸の鎖数: 24
• 化学式量合計: 1167745.39

構造登録者

Li, F.,Cingolani, G.,Hou, C. (登録日: 2021-10-04, 公開日: 2022-11-09, 最終更新日: 2024-06-05)

主引用文献

Li, F.,Hou, C.D.,Yang, R.,Whitehead III, R.,Teschke, C.M.,Cingolani, G.
High-resolution cryo-EM structure of the Shigella virus Sf6 genome delivery tail machine.
Sci Adv, 8:eadc9641-eadc9641, 2022

PubMed Abstract: Sf6 is a bacterial virus that infects the human pathogen Here, we describe the cryo-electron microscopy structure of the Sf6 tail machine before DNA ejection, which we determined at a 2.7-angstrom resolution. We built de novo structures of all tail components and resolved four symmetry-mismatched interfaces. Unexpectedly, we found that the tail exists in two conformations, rotated by ~6° with respect to the capsid. The two tail conformers are identical in structure but differ solely in how the portal and head-to-tail adaptor carboxyl termini bond with the capsid at the fivefold vertex, similar to a diamond held over a five-pronged ring in two nonidentical states. Thus, in the mature Sf6 tail, the portal structure does not morph locally to accommodate the symmetry mismatch but exists in two energetic minima rotated by a discrete angle. We propose that the design principles of the Sf6 tail are conserved across P22-like Podoviridae.

PubMed: 36475795
DOI: 10.1126/sciadv.adc9641

実験手法

ELECTRON MICROSCOPY (2.76 Å)