7SF9
Branchiostoma Floridae Violet Fluorescent Protein
Summary for 7SF9
| Entry DOI | 10.2210/pdb7sf9/pdb |
| Descriptor | Violet Fluorescent Protein (2 entities in total) |
| Functional Keywords | fluorescent protein, oxidized tyrosine, chromophore |
| Biological source | Branchiostoma floridae (Florida lancelet, Amphioxus) |
| Total number of polymer chains | 4 |
| Total formula weight | 107697.24 |
| Authors | Pletnev, S.,Pletneva, N.,Pletnev, V.Z.,Muslinkina, L. (deposition date: 2021-10-03, release date: 2022-03-30, Last modification date: 2023-10-18) |
| Primary citation | Roldan-Salgado, A.,Muslinkina, L.,Pletnev, S.,Pletneva, N.,Pletnev, V.,Gaytan, P. A novel violet fluorescent protein contains a unique oxidized tyrosine as the simplest chromophore ever reported in fluorescent proteins. Protein Sci., 31:688-700, 2022 Cited by PubMed Abstract: We describe an engineered violet fluorescent protein from the lancelet Branchiostoma floridae (bfVFP). This is the first example of a GFP-like fluorescent protein with a stable fluorescent chromophore lacking an imidazolinone ring; instead, it consists of oxidized tyrosine 68 flanked by glycine 67 and alanine 69. bfVFP contains the simplest chromophore reported in fluorescent proteins and was generated from the yellow protein lanFP10A2 by two synergetic mutations, S148H and C166I. The chromophore structure was confirmed crystallographically and by high-resolution mass spectrometry. The photophysical characteristics of bfVFP (323/430 nm, quantum yield 0.33, and E 14,300 M cm ) make it potentially useful for multicolor experiments to expand the excitation range of available FP biomarkers and Förster resonance energy transfer with blue and cyan fluorescent protein acceptors. PubMed: 34936154DOI: 10.1002/pro.4265 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.8 Å) |
Structure validation
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