7SF9

Branchiostoma Floridae Violet Fluorescent Protein

7SF9 の概要

• エントリーDOI: 10.2210/pdb7sf9/pdb
• 分子名称: Violet Fluorescent Protein (2 entities in total)
• 機能のキーワード: fluorescent protein, oxidized tyrosine, chromophore
• 由来する生物種: Branchiostoma floridae (Florida lancelet, Amphioxus)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 107697.24

構造登録者

Pletnev, S.,Pletneva, N.,Pletnev, V.Z.,Muslinkina, L. (登録日: 2021-10-03, 公開日: 2022-03-30, 最終更新日: 2023-10-18)

主引用文献

Roldan-Salgado, A.,Muslinkina, L.,Pletnev, S.,Pletneva, N.,Pletnev, V.,Gaytan, P.
A novel violet fluorescent protein contains a unique oxidized tyrosine as the simplest chromophore ever reported in fluorescent proteins.
Protein Sci., 31:688-700, 2022

PubMed Abstract: We describe an engineered violet fluorescent protein from the lancelet Branchiostoma floridae (bfVFP). This is the first example of a GFP-like fluorescent protein with a stable fluorescent chromophore lacking an imidazolinone ring; instead, it consists of oxidized tyrosine 68 flanked by glycine 67 and alanine 69. bfVFP contains the simplest chromophore reported in fluorescent proteins and was generated from the yellow protein lanFP10A2 by two synergetic mutations, S148H and C166I. The chromophore structure was confirmed crystallographically and by high-resolution mass spectrometry. The photophysical characteristics of bfVFP (323/430 nm, quantum yield 0.33, and E 14,300 M  cm ) make it potentially useful for multicolor experiments to expand the excitation range of available FP biomarkers and Förster resonance energy transfer with blue and cyan fluorescent protein acceptors.

PubMed: 34936154
DOI: 10.1002/pro.4265

実験手法

X-RAY DIFFRACTION (1.8 Å)