7SCZ

Nuc147 bound to multiple BRCTs

7SCZ の概要

• エントリーDOI: 10.2210/pdb7scz/pdb
• EMDBエントリー: 25043
• 分子名称: DNA (147-MER), Histone H3.1, Histone H4, ... (7 entities in total)
• 機能のキーワード: parp1, brct, nucleosome, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 11
• 化学式量合計: 217209.34

構造登録者

Muthurajan, U.M.,Rudolph, J. (登録日: 2021-09-29, 公開日: 2022-01-19, 最終更新日: 2024-06-05)

主引用文献

Rudolph, J.,Muthurajan, U.M.,Palacio, M.,Mahadevan, J.,Roberts, G.,Erbse, A.H.,Dyer, P.N.,Luger, K.
The BRCT domain of PARP1 binds intact DNA and mediates intrastrand transfer.
Mol.Cell, 81:4994-5006.e5, 2021

PubMed Abstract: PARP1 is a key player in the response to DNA damage and is the target of clinical inhibitors for the treatment of cancers. Binding of PARP1 to damaged DNA leads to activation wherein PARP1 uses NAD to add chains of poly(ADP-ribose) onto itself and other nuclear proteins. PARP1 also binds abundantly to intact DNA and chromatin, where it remains enzymatically inactive. We show that intact DNA makes contacts with the PARP1 BRCT domain, which was not previously recognized as a DNA-binding domain. This binding mode does not result in the concomitant reorganization and activation of the catalytic domain. We visualize the BRCT domain bound to nucleosomal DNA by cryogenic electron microscopy and identify a key motif conserved from ancestral BRCT domains for binding phosphates on DNA and phospho-peptides. Finally, we demonstrate that the DNA-binding properties of the BRCT domain contribute to the "monkey-bar mechanism" that mediates DNA transfer of PARP1.

PubMed: 34919819
DOI: 10.1016/j.molcel.2021.11.014

実験手法

ELECTRON MICROSCOPY (3.5 Å)