7SCQ

tRNA-like Structure from Brome Mosaic Virus Bound to Tyrosyl-tRNA Synthetase from Phaseolus vulgaris. Conformation: Bound State 2.

Summary for 7SCQ

• Entry DOI: 10.2210/pdb7scq/pdb
• Related: 7SAM 7SC6
• EMDB information: 25041
• Descriptor: Tyrosine--tRNA ligase, tRNA-like structure from brome mosaic virus RNA 3. (2 entities in total)
• Functional Keywords: trna-synthetase, rna-protein complex, viral rna, 3' utr, rna binding protein, rna binding protein-rna complex, rna binding protein/rna
• Biological source: Phaseolus vulgaris (Kidney bean, French bean); More
• Total number of polymer chains: 3
• Total formula weight: 146008.94

Authors

Kieft, J.S.,Bonilla, S.L. (deposition date: 2021-09-28, release date: 2021-12-01, Last modification date: 2024-06-05)

Primary citation

Bonilla, S.L.,Sherlock, M.E.,MacFadden, A.,Kieft, J.S.
A viral RNA hijacks host machinery using dynamic conformational changes of a tRNA-like structure.
Science, 374:955-960, 2021

PubMed Abstract: Viruses require multifunctional structured RNAs to hijack their host’s biochemistry, but their mechanisms can be obscured by the difficulty of solving conformationally dynamic RNA structures. Using cryo–electron microscopy (cryo-EM), we visualized the structure of the mysterious viral transfer RNA (tRNA)–like structure (TLS) from the brome mosaic virus, which affects replication, translation, and genome encapsidation. Structures in isolation and those bound to tyrosyl-tRNA synthetase (TyrRS) show that this ~55-kilodalton purported tRNA mimic undergoes large conformational rearrangements to bind TyrRS in a form that differs substantially from that of tRNA. Our study reveals how viral RNAs can use a combination of static and dynamic RNA structures to bind host machinery through highly noncanonical interactions, and we highlight the utility of cryo-EM for visualizing small, conformationally dynamic structured RNAs.

PubMed: 34793227
DOI: 10.1126/science.abe8526

Experimental method

ELECTRON MICROSCOPY (6 Å)