7SAM
tRNA-like Structure from Brome Mosaic Virus
Summary for 7SAM
| Entry DOI | 10.2210/pdb7sam/pdb |
| EMDB information | 24952 |
| Descriptor | Viral RNA (1 entity in total) |
| Functional Keywords | viral rna, 3' utr, rna |
| Biological source | Brome mosaic virus |
| Total number of polymer chains | 1 |
| Total formula weight | 55044.56 |
| Authors | Kieft, J.S.,Bonilla, S.L. (deposition date: 2021-09-22, release date: 2021-12-01, Last modification date: 2024-06-05) |
| Primary citation | Bonilla, S.L.,Sherlock, M.E.,MacFadden, A.,Kieft, J.S. A viral RNA hijacks host machinery using dynamic conformational changes of a tRNA-like structure. Science, 374:955-960, 2021 Cited by PubMed Abstract: Viruses require multifunctional structured RNAs to hijack their host’s biochemistry, but their mechanisms can be obscured by the difficulty of solving conformationally dynamic RNA structures. Using cryo–electron microscopy (cryo-EM), we visualized the structure of the mysterious viral transfer RNA (tRNA)–like structure (TLS) from the brome mosaic virus, which affects replication, translation, and genome encapsidation. Structures in isolation and those bound to tyrosyl-tRNA synthetase (TyrRS) show that this ~55-kilodalton purported tRNA mimic undergoes large conformational rearrangements to bind TyrRS in a form that differs substantially from that of tRNA. Our study reveals how viral RNAs can use a combination of static and dynamic RNA structures to bind host machinery through highly noncanonical interactions, and we highlight the utility of cryo-EM for visualizing small, conformationally dynamic structured RNAs. PubMed: 34793227DOI: 10.1126/science.abe8526 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (4.3 Å) |
Structure validation
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