7SAM

tRNA-like Structure from Brome Mosaic Virus

7SAM の概要

• エントリーDOI: 10.2210/pdb7sam/pdb
• EMDBエントリー: 24952
• 分子名称: Viral RNA (1 entity in total)
• 機能のキーワード: viral rna, 3' utr, rna
• 由来する生物種: Brome mosaic virus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 55044.56

構造登録者

Kieft, J.S.,Bonilla, S.L. (登録日: 2021-09-22, 公開日: 2021-12-01, 最終更新日: 2024-06-05)

主引用文献

Bonilla, S.L.,Sherlock, M.E.,MacFadden, A.,Kieft, J.S.
A viral RNA hijacks host machinery using dynamic conformational changes of a tRNA-like structure.
Science, 374:955-960, 2021

PubMed Abstract: Viruses require multifunctional structured RNAs to hijack their host’s biochemistry, but their mechanisms can be obscured by the difficulty of solving conformationally dynamic RNA structures. Using cryo–electron microscopy (cryo-EM), we visualized the structure of the mysterious viral transfer RNA (tRNA)–like structure (TLS) from the brome mosaic virus, which affects replication, translation, and genome encapsidation. Structures in isolation and those bound to tyrosyl-tRNA synthetase (TyrRS) show that this ~55-kilodalton purported tRNA mimic undergoes large conformational rearrangements to bind TyrRS in a form that differs substantially from that of tRNA. Our study reveals how viral RNAs can use a combination of static and dynamic RNA structures to bind host machinery through highly noncanonical interactions, and we highlight the utility of cryo-EM for visualizing small, conformationally dynamic structured RNAs.

PubMed: 34793227
DOI: 10.1126/science.abe8526

実験手法

ELECTRON MICROSCOPY (4.3 Å)