7SA1

LRR-F-Box plant ubiquitin ligase

7SA1 の概要

• エントリーDOI: 10.2210/pdb7sa1/pdb
• 分子名称: SKP1-like protein 1A, F-box/LRR-repeat MAX2 homolog, DI(HYDROXYETHYL)ETHER, ... (5 entities in total)
• 機能のキーワード: rice d3, ask1, e3-ub ligase, signaling protein
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 188246.85

構造登録者

Palayam, M.,Shabek, N. (登録日: 2021-09-21, 公開日: 2022-04-20, 最終更新日: 2023-10-18)

主引用文献

Tal, L.,Palayam, M.,Ron, M.,Young, A.,Britt, A.,Shabek, N.
A conformational switch in the SCF-D3/MAX2 ubiquitin ligase facilitates strigolactone signalling.
Nat.Plants, 8:561-573, 2022

PubMed Abstract: Strigolactones (SLs) are a class of plant hormones that regulate numerous processes of growth and development. SL perception and signal activation involves interaction between F-box E3 ubiquitin ligase D3/MAX2 and DWARF14 (D14) α/β-hydrolase in a SL-dependent manner and targeting of D53/SMXL6/7/8 transcriptional repressors (SMXLs) for proteasome-mediated degradation. D3/MAX2 has been shown to exist in multiple conformational states in which the C-terminal helix (CTH) undergoes a closed-to-open dynamics and regulates D14 binding and SL perception. Despite the multiple modes of D3-D14 interactions found in vitro, the residues that regulate the conformational switch of D3/MAX2 CTH in targeting D53/SMXLs and the subsequent effect on SL signalling remain unclear. Here we elucidate the functional dynamics of ASK1-D3/MAX2 in SL signalling by leveraging conformational switch mutants in vitro and in plants. We report the crystal structure of a dislodged CTH of the ASK1-D3 mutant and demonstrate that disruptions in CTH plasticity via either CRISPR-Cas9 genome editing or expression of point mutation mutants result in impairment of SL signalling. We show that the conformational switch in ASK1-D3/MAX2 CTH directly regulates ubiquitin-mediated protein degradation. A dislodged conformation involved in D53/SMXLs SL-dependent recruitment and ubiquitination and an engaged conformation are required for the release of polyubiquitinated D53/SMXLs and subsequently D14 for proteasomal degradation. Finally, we uncovered an organic acid metabolite that can directly trigger the D3/MAX2 CTH conformational switch. Our findings unravel a new regulatory function of a SKP1-CUL1-F-box ubiquitin ligase in plant signalling.

PubMed: 35484202
DOI: 10.1038/s41477-022-01145-7

実験手法

X-RAY DIFFRACTION (3.21 Å)