7S4Z

Serial Macromolecular Crystallography at ALBA Synchrotron Light Source - Proteinase K

7S4Z の概要

• エントリーDOI: 10.2210/pdb7s4z/pdb
• 関連するPDBエントリー: 7S4R 7S4W 7S4Y
• 分子名称: Proteinase K, NITRATE ION, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: serial crystallography, lcp injection, hydrolase
• 由来する生物種: Parengyodontium album
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29100.95

構造登録者

Martin-Garcia, J.M.,Botha, S.,Hu, H.,Jernigan, R.,Castellvi, A.,Lisova, S.,Gil, F.,Calisto, B.,Crespo, I.,Roy-Chowdbury, S.,Grieco, A.,Ketawala, G.,Weierstall, U.,Spence, J.,Fromme, P.,Zatsepin, N.,Boer, R.,Carpena, X. (登録日: 2021-09-09, 公開日: 2021-10-27, 最終更新日: 2024-10-30)

主引用文献

Martin-Garcia, J.M.,Botha, S.,Hu, H.,Jernigan, R.,Castellvi, A.,Lisova, S.,Gil, F.,Calisto, B.,Crespo, I.,Roy-Chowdhury, S.,Grieco, A.,Ketawala, G.,Weierstall, U.,Spence, J.,Fromme, P.,Zatsepin, N.,Boer, D.R.,Carpena, X.
Serial macromolecular crystallography at ALBA Synchrotron Light Source.
J.Synchrotron Radiat., 29:896-907, 2022

PubMed Abstract: The increase in successful adaptations of serial crystallography at synchrotron radiation sources continues. To date, the number of serial synchrotron crystallography (SSX) experiments has grown exponentially, with over 40 experiments reported so far. In this work, we report the first SSX experiments with viscous jets conducted at ALBA beamline BL13-XALOC. Small crystals (15-30 µm) of five soluble proteins (lysozyme, proteinase K, phycocyanin, insulin and α-spectrin-SH3 domain) were suspended in lipidic cubic phase (LCP) and delivered to the X-ray beam with a high-viscosity injector developed at Arizona State University. Complete data sets were collected from all proteins and their high-resolution structures determined. The high quality of the diffraction data collected from all five samples, and the lack of specific radiation damage in the structures obtained in this study, confirm that the current capabilities at the beamline enables atomic resolution determination of protein structures from microcrystals as small as 15 µm using viscous jets at room temperature. Thus, BL13-XALOC can provide a feasible alternative to X-ray free-electron lasers when determining snapshots of macromolecular structures.

PubMed: 35511023
DOI: 10.1107/S1600577522002508

実験手法

X-RAY DIFFRACTION (1.9 Å)