7S20

M. xanthus encapsulin shell protein EncA with T=3 symmetry

7S20 の概要

• エントリーDOI: 10.2210/pdb7s20/pdb
• EMDBエントリー: 24814
• 分子名称: EncA (1 entity in total)
• 機能のキーワード: nanocage, encapsulin, iron storage, bacterial nano-compartment, cytosolic protein, virus like particle
• 由来する生物種: Myxococcus xanthus
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 100515.22

構造登録者

Eren, E. (登録日: 2021-09-02, 公開日: 2022-02-02, 最終更新日: 2024-06-05)

主引用文献

Eren, E.,Wang, B.,Winkler, D.C.,Watts, N.R.,Steven, A.C.,Wingfield, P.T.
Structural characterization of the Myxococcus xanthus encapsulin and ferritin-like cargo system gives insight into its iron storage mechanism.
Structure, 30:551-563.e4, 2022

PubMed Abstract: Encapsulins are bacterial organelle-like cages involved in various aspects of metabolism, especially protection from oxidative stress. They can serve as vehicles for a wide range of medical applications. Encapsulin shell proteins are structurally similar to HK97 bacteriophage capsid protein and their function depends on the encapsulated cargos. The Myxococcus xanthus encapsulin system comprises EncA and three cargos: EncB, EncC, and EncD. EncB and EncC are similar to bacterial ferritins that can oxidize Fe to less toxic Fe. We analyzed EncA, EncB, and EncC by cryo-EM and X-ray crystallography. Cryo-EM shows that EncA cages can have T = 3 and T = 1 symmetry and that EncA T = 1 has a unique protomer arrangement. Also, we define EncB and EncC binding sites on EncA. X-ray crystallography of EncB and EncC reveals conformational changes at the ferroxidase center and additional metal binding sites, suggesting a mechanism for Fe oxidation and storage within the encapsulin shell.

PubMed: 35150605
DOI: 10.1016/j.str.2022.01.008

実験手法

ELECTRON MICROSCOPY (3.4 Å)