7RYS

SthK R120A Open State 2

Summary for 7RYS

• Entry DOI: 10.2210/pdb7rys/pdb
• EMDB information: 24747
• Descriptor: SthK, ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE, (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate (3 entities in total)
• Functional Keywords: cyclic nucleotide-gated ion channel, transport protein
• Biological source: Spirochaeta thermophila
• Total number of polymer chains: 4
• Total formula weight: 226418.86

Authors

Gao, X.,Nimigean, C.,Schmidpeter, P. (deposition date: 2021-08-26, release date: 2022-11-23, Last modification date: 2024-06-05)

Primary citation

Gao, X.,Schmidpeter, P.A.M.,Berka, V.,Durham, R.J.,Fan, C.,Jayaraman, V.,Nimigean, C.M.
Gating intermediates reveal inhibitory role of the voltage sensor in a cyclic nucleotide-modulated ion channel.
Nat Commun, 13:6919-6919, 2022

PubMed Abstract: Understanding how ion channels gate is important for elucidating their physiological roles and targeting them in pathophysiological states. Here, we used SthK, a cyclic nucleotide-modulated channel from Spirochaeta thermophila, to define a ligand-gating trajectory that includes multiple on-pathway intermediates. cAMP is a poor partial agonist for SthK and depolarization increases SthK activity. Tuning the energy landscape by gain-of-function mutations in the voltage sensor domain (VSD) allowed us to capture multiple intermediates along the ligand-activation pathway, highlighting the allosteric linkage between VSD, cyclic nucleotide-binding (CNBD) and pore domains. Small, lateral displacements of the VSD S4 segment were necessary to open the intracellular gate, pointing to an inhibitory VSD at rest. We propose that in wild-type SthK, depolarization leads to such VSD displacements resulting in release of inhibition. In summary, we report conformational transitions along the activation pathway that reveal allosteric couplings between key sites integrating to open the intracellular gate.

PubMed: 36376326
DOI: 10.1038/s41467-022-34673-z

Experimental method

ELECTRON MICROSCOPY (3.7 Å)