7RYE

Cryo-EM structure of the needle filament-tip complex of the Salmonella type III secretion injectisome

7RYE の概要

• エントリーDOI: 10.2210/pdb7rye/pdb
• EMDBエントリー: 24735
• 分子名称: Protein PrgI, Cell invasion protein SipD (2 entities in total)
• 機能のキーワード: protein secretion, bacterial pathogenesis, organelle assembly, cell invasion
• 由来する生物種: Salmonella enterica subsp. enterica serovar Typhimurium; 詳細
• タンパク質・核酸の鎖数: 24
• 化学式量合計: 354138.23

構造登録者

Guo, E.Z.,Galan, J.E. (登録日: 2021-08-25, 公開日: 2021-11-10, 最終更新日: 2025-05-28)

主引用文献

Guo, E.Z.,Galan, J.E.
Cryo-EM structure of the needle filament tip complex of the Salmonella type III secretion injectisome.
Proc.Natl.Acad.Sci.USA, 118:-, 2021

PubMed Abstract: Type III secretion systems are multiprotein molecular machines required for the virulence of several important bacterial pathogens. The central element of these machines is the injectisome, a ∼5-Md multiprotein structure that mediates the delivery of bacterially encoded proteins into eukaryotic target cells. The injectisome is composed of a cytoplasmic sorting platform, and a membrane-embedded needle complex, which is made up of a multiring base and a needle-like filament that extends several nanometers from the bacterial surface. The needle filament is capped at its distal end by another substructure known as the tip complex, which is crucial for the translocation of effector proteins through the eukaryotic cell plasma membrane. Here we report the cryo-EM structure of the Typhimurium needle tip complex docked onto the needle filament tip. Combined with a detailed analysis of structurally guided mutants, this study provides major insight into the assembly and function of this essential component of the type III secretion protein injection machine.

PubMed: 34706941
DOI: 10.1073/pnas.2114552118

実験手法

ELECTRON MICROSCOPY (3.9 Å)