7RX4

Cryo-EM reconstruction of AS2 nanotube (Form II like)

7RX4 の概要

• エントリーDOI: 10.2210/pdb7rx4/pdb
• EMDBエントリー: 24724
• 分子名称: AS2 peptide (1 entity in total)
• 機能のキーワード: helical symmetry, peptide nanotube, self-assembly, structural protein
• 由来する生物種: Synthetic construct
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 6449.34

構造登録者

Wang, F.,Gnewou, O.M.,Solemanifar, A.,Xu, C.,Egelman, E.H.,Conticello, V.P. (登録日: 2021-08-21, 公開日: 2021-09-08, 最終更新日: 2024-06-05)

主引用文献

Wang, F.,Gnewou, O.,Solemanifar, A.,Conticello, V.P.,Egelman, E.H.
Cryo-EM of Helical Polymers.
Chem.Rev., 122:14055-14065, 2022

PubMed Abstract: While the application of cryogenic electron microscopy (cryo-EM) to helical polymers in biology has a long history, due to the huge number of helical macromolecular assemblies in viruses, bacteria, archaea, and eukaryotes, the use of cryo-EM to study synthetic soft matter noncovalent polymers has been much more limited. This has mainly been due to the lack of familiarity with cryo-EM in the materials science and chemistry communities, in contrast to the fact that cryo-EM was developed as a biological technique. Nevertheless, the relatively few structures of self-assembled peptide nanotubes and ribbons solved at near-atomic resolution by cryo-EM have demonstrated that cryo-EM should be the method of choice for a structural analysis of synthetic helical filaments. In addition, cryo-EM has also demonstrated that the self-assembly of soft matter polymers has enormous potential for polymorphism, something that may be obscured by techniques such as scattering and spectroscopy. These cryo-EM structures have revealed how far we currently are from being able to predict the structure of these polymers due to their chaotic self-assembly behavior.

PubMed: 35133794
DOI: 10.1021/acs.chemrev.1c00753

実験手法

ELECTRON MICROSCOPY (3.8 Å)