7RWU

Crystal structure of Ni-bound RIDC1 variant in the presence of reductant

7RWU の概要

• エントリーDOI: 10.2210/pdb7rwu/pdb
• 分子名称: Soluble cytochrome b562, NICKEL (II) ION, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, ... (6 entities in total)
• 機能のキーワード: metal binding protein
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12609.29

構造登録者

Kakkis, A.,Golub, E. (登録日: 2021-08-20, 公開日: 2022-06-29, 最終更新日: 2023-10-25)

主引用文献

Kakkis, A.,Golub, E.,Choi, T.S.,Tezcan, F.A.
Redox- and metal-directed structural diversification in designed metalloprotein assemblies.
Chem.Commun.(Camb.), 58:6958-6961, 2022

PubMed Abstract: Herein we describe a designed protein building block whose self-assembly behaviour is dually gated by the redox state of disulphide bonds and the identity of exogenous metal ions. This protein construct is shown - through extensive structural and biophysical characterization - to access five distinct oligomeric states, exemplifying how the complex interplay between hydrophobic, metal-ligand, and reversible covalent interactions could be harnessed to obtain multiple, responsive protein architectures from a single building block.

PubMed: 35642584
DOI: 10.1039/d2cc02440c

実験手法

X-RAY DIFFRACTION (1.8 Å)