7RWS

Structure of SAVED domain of Cap5 from Lactococcus lactis in complex with cGAMP

7RWS の概要

• エントリーDOI: 10.2210/pdb7rws/pdb
• 分子名称: SAVED domain-containing protein, 3'2'-cGAMP (3 entities in total)
• 機能のキーワード: saved sensor cgamp second messenger, unknown function
• 由来する生物種: Lactococcus lactis subsp. cremoris IBB477
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 31288.06

構造登録者

Huang, R.H.,Chakravarti, A.,Fatma, S. (登録日: 2021-08-20, 公開日: 2021-11-10, 最終更新日: 2023-10-18)

主引用文献

Fatma, S.,Chakravarti, A.,Zeng, X.,Huang, R.H.
Molecular mechanisms of the CdnG-Cap5 antiphage defense system employing 3',2'-cGAMP as the second messenger.
Nat Commun, 12:6381-6381, 2021

PubMed Abstract: Cyclic-oligonucleotide-based antiphage signaling systems (CBASS) are diverse and abundant in bacteria. Here, we present the biochemical and structural characterization of two CBASS systems, composed of CdnG and Cap5, from Asticcacaulis sp. and Lactococcus lactis. We show that CdnG from Asticcacaulis sp. synthesizes 3',2'-cGAMP in vitro, and 3',2'-cGAMP is the biological signaling molecule that activates Cap5 for DNA degradation. Crystal structures of Cap5, together with the SAVED domain in complex with 3',2'-cGAMP, provide insight into the architecture of Cap5 as well as molecular recognition of 3',2'-cGAMP by the SAVED domain of Cap5. Amino acid conservation of the SAVED domain of Cap5, together with mutational studies, led us to propose a mechanism of Back-to-Front stacking of two SAVED domains, mediated by 3',2'-cGAMP, to activate HNH nuclease domain for DNA degradation. This study of the most abundant CBASS system provides insights into the mechanisms employed by bacteria in their conflicts against phage.

PubMed: 34737303
DOI: 10.1038/s41467-021-26738-2

実験手法

X-RAY DIFFRACTION (1.8 Å)