Summary for 7RWL
| Entry DOI | 10.2210/pdb7rwl/pdb |
| EMDB information | 24718 24719 |
| Descriptor | Capsid protein VP1 (1 entity in total) |
| Functional Keywords | exo-aav, exosome-associated adeno-associated virus, ea-aav, envelope-associated adeno-associated virus, virus |
| Biological source | Adeno-associated dependoparvovirus A |
| Total number of polymer chains | 60 |
| Total formula weight | 4921881.12 |
| Authors | Hull, J.A.,Mietzsch, M.,Chipman, P.,Strugatsky, D.,McKenna, R. (deposition date: 2021-08-20, release date: 2021-10-20, Last modification date: 2025-05-14) |
| Primary citation | Hull, J.A.,Mietzsch, M.,Chipman, P.,Strugatsky, D.,McKenna, R. Structural characterization of an envelope-associated adeno-associated virus type 2 capsid. Virology, 565:22-28, 2021 Cited by PubMed Abstract: Adeno-associated virus (AAV) are classified as non-enveloped ssDNA viruses. However, AAV capsids embedded within exosomes have been observed, and it has been suggested that the AAV membrane associated accessory protein (MAAP) may play a role in envelope-associated AAV (EA-AAV) capsid formation. Here, we observed and selected sufficient homogeneous EA-AAV capsids of AAV2, produced using the Sf9 baculoviral expression system, to determine the cryo-electron microscopy (cryo-EM) structure at 3.14 Å resolution. The reconstructed map confirmed that the EA-AAV capsid, showed no significant structural variation compared to the non-envelope capsid. In addition, the Sf9 expression system used implies the notion that MAAP may enhance exosome AAV encapsulation. Furthermore, we speculate that these EA-AAV capsids may have therapeutic benefits over the currently used non-envelope AAV capsids, with advantages in immune evasion and/or improved infectivity. PubMed: 34638006DOI: 10.1016/j.virol.2021.09.010 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.14 Å) |
Structure validation
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