7RWL

Envelope-associated Adeno-associated virus serotype 2

これはPDB形式変換不可エントリーです。

7RWL の概要

• エントリーDOI: 10.2210/pdb7rwl/pdb
• EMDBエントリー: 24718 24719
• 分子名称: Capsid protein VP1 (1 entity in total)
• 機能のキーワード: exo-aav, exosome-associated adeno-associated virus, ea-aav, envelope-associated adeno-associated virus, virus
• 由来する生物種: Adeno-associated dependoparvovirus A
• タンパク質・核酸の鎖数: 60
• 化学式量合計: 4921881.12

構造登録者

Hull, J.A.,Mietzsch, M.,Chipman, P.,Strugatsky, D.,McKenna, R. (登録日: 2021-08-20, 公開日: 2021-10-20, 最終更新日: 2025-05-14)

主引用文献

Hull, J.A.,Mietzsch, M.,Chipman, P.,Strugatsky, D.,McKenna, R.
Structural characterization of an envelope-associated adeno-associated virus type 2 capsid.
Virology, 565:22-28, 2021

PubMed Abstract: Adeno-associated virus (AAV) are classified as non-enveloped ssDNA viruses. However, AAV capsids embedded within exosomes have been observed, and it has been suggested that the AAV membrane associated accessory protein (MAAP) may play a role in envelope-associated AAV (EA-AAV) capsid formation. Here, we observed and selected sufficient homogeneous EA-AAV capsids of AAV2, produced using the Sf9 baculoviral expression system, to determine the cryo-electron microscopy (cryo-EM) structure at 3.14 Å resolution. The reconstructed map confirmed that the EA-AAV capsid, showed no significant structural variation compared to the non-envelope capsid. In addition, the Sf9 expression system used implies the notion that MAAP may enhance exosome AAV encapsulation. Furthermore, we speculate that these EA-AAV capsids may have therapeutic benefits over the currently used non-envelope AAV capsids, with advantages in immune evasion and/or improved infectivity.

PubMed: 34638006
DOI: 10.1016/j.virol.2021.09.010

実験手法

ELECTRON MICROSCOPY (3.14 Å)