7RVB

High resolution map of molecular chaperone Artemin

7RVB の概要

• エントリーDOI: 10.2210/pdb7rvb/pdb
• EMDBエントリー: 24706 24707
• 分子名称: Ferritin (1 entity in total)
• 機能のキーワード: molecular chaperone artemin is a homolog of apoferritin, chaperone
• 由来する生物種: Artemia franciscana
• タンパク質・核酸の鎖数: 24
• 化学式量合計: 627477.89

構造登録者

Parvate, A.D.,Powell, S.M.,Brookreason, J.T.,Novikova, I.V.,Evans, J.E. (登録日: 2021-08-18, 公開日: 2022-12-14, 最終更新日: 2024-11-06)

主引用文献

Parvate, A.D.,Powell, S.M.,Brookreson, J.T.,Moser, T.H.,Novikova, I.V.,Zhou, M.,Evans, J.E.
Cryo-EM structure of the diapause chaperone artemin.
Front Mol Biosci, 9:998562-998562, 2022

PubMed Abstract: The protein artemin acts as both an RNA and protein chaperone and constitutes over 10% of all protein in cysts during diapause. However, its mechanistic details remain elusive since no high-resolution structure of artemin exists. Here we report the full-length structure of artemin at 2.04 Å resolution. The cryo-EM map contains density for an intramolecular disulfide bond between Cys22-Cys61 and resolves the entire C-terminus extending into the core of the assembled protein cage but in a different configuration than previously hypothesized with molecular modeling. We also provide data supporting the role of C-terminal helix F towards stabilizing the dimer form that is believed to be important for its chaperoning activity. We were able to destabilize this effect by placing a tag at the C-terminus to fully pack the internal cavity and cause limited steric hindrance.

PubMed: 36518848
DOI: 10.3389/fmolb.2022.998562

実験手法

ELECTRON MICROSCOPY (2.04 Å)