7RTE

X-ray structure of wild type RBPJ-L3MBTL3-DNA complex

これはPDB形式変換不可エントリーです。

7RTE の概要

• エントリーDOI: 10.2210/pdb7rte/pdb
• 分子名称: DNA (5'-D(*AP*AP*TP*CP*TP*TP*TP*CP*CP*CP*AP*CP*GP*GP*T)-3'), DNA (5'-D(*TP*TP*AP*CP*CP*GP*TP*GP*GP*GP*AP*AP*AP*GP*A)-3'), Recombining binding protein suppressor of hairless, ... (7 entities in total)
• 機能のキーワード: notch signaling, transcription, rbpj, csl, l3mbtl3, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 59041.89

構造登録者

Hall, D.P.,Kovall, R.A.,Yuan, Z. (登録日: 2021-08-13, 公開日: 2022-08-24, 最終更新日: 2023-10-25)

主引用文献

Hall, D.,Giaimo, B.D.,Park, S.S.,Hemmer, W.,Friedrich, T.,Ferrante, F.,Bartkuhn, M.,Yuan, Z.,Oswald, F.,Borggrefe, T.,Rual, J.F.,Kovall, R.A.
The structure, binding and function of a Notch transcription complex involving RBPJ and the epigenetic reader protein L3MBTL3.
Nucleic Acids Res., 50:13083-13099, 2022

PubMed Abstract: The Notch pathway transmits signals between neighboring cells to elicit downstream transcriptional programs. Notch is a major regulator of cell fate specification, proliferation, and apoptosis, such that aberrant signaling leads to a pleiotropy of human diseases, including developmental disorders and cancers. The pathway signals through the transcription factor CSL (RBPJ in mammals), which forms an activation complex with the intracellular domain of the Notch receptor and the coactivator Mastermind. CSL can also function as a transcriptional repressor by forming complexes with one of several different corepressor proteins, such as FHL1 or SHARP in mammals and Hairless in Drosophila. Recently, we identified L3MBTL3 as a bona fide RBPJ-binding corepressor that recruits the repressive lysine demethylase LSD1/KDM1A to Notch target genes. Here, we define the RBPJ-interacting domain of L3MBTL3 and report the 2.06 Å crystal structure of the RBPJ-L3MBTL3-DNA complex. The structure reveals that L3MBTL3 interacts with RBPJ via an unusual binding motif compared to other RBPJ binding partners, which we comprehensively analyze with a series of structure-based mutants. We also show that these disruptive mutations affect RBPJ and L3MBTL3 function in cells, providing further insights into Notch mediated transcriptional regulation.

PubMed: 36477367
DOI: 10.1093/nar/gkac1137

実験手法

X-RAY DIFFRACTION (2.06 Å)