7RRO

Structure of the 48-nm repeat doublet microtubule from bovine tracheal cilia

これはPDB形式変換不可エントリーです。

7RRO の概要

• エントリーDOI: 10.2210/pdb7rro/pdb
• EMDBエントリー: 24663 24664
• 分子名称: Protein C9orf135 homolog, Tektin-2, Uncharacterized protein C1orf189 homolog, ... (39 entities in total)
• 機能のキーワード: cilia, microtubule, dynein, motility, structural protein
• 由来する生物種: Bos taurus (Bovine); 詳細
• タンパク質・核酸の鎖数: 429
• 化学式量合計: 21608302.42

構造登録者

Gui, M.,Anderson, J.R.,Botsch, J.J.,Meleppattu, S.,Singh, S.K.,Zhang, Q.,Brown, A. (登録日: 2021-08-10, 公開日: 2021-10-27, 最終更新日: 2024-06-05)

主引用文献

Gui, M.,Farley, H.,Anujan, P.,Anderson, J.R.,Maxwell, D.W.,Whitchurch, J.B.,Botsch, J.J.,Qiu, T.,Meleppattu, S.,Singh, S.K.,Zhang, Q.,Thompson, J.,Lucas, J.S.,Bingle, C.D.,Norris, D.P.,Roy, S.,Brown, A.
De novo identification of mammalian ciliary motility proteins using cryo-EM.
Cell, 184:5791-, 2021

PubMed Abstract: Dynein-decorated doublet microtubules (DMTs) are critical components of the oscillatory molecular machine of cilia, the axoneme, and have luminal surfaces patterned periodically by microtubule inner proteins (MIPs). Here we present an atomic model of the 48-nm repeat of a mammalian DMT, derived from a cryoelectron microscopy (cryo-EM) map of the complex isolated from bovine respiratory cilia. The structure uncovers principles of doublet microtubule organization and features specific to vertebrate cilia, including previously unknown MIPs, a luminal bundle of tektin filaments, and a pentameric dynein-docking complex. We identify a mechanism for bridging 48- to 24-nm periodicity across the microtubule wall and show that loss of the proteins involved causes defective ciliary motility and laterality abnormalities in zebrafish and mice. Our structure identifies candidate genes for diagnosis of ciliopathies and provides a framework to understand their functions in driving ciliary motility.

PubMed: 34715025
DOI: 10.1016/j.cell.2021.10.007

実験手法

ELECTRON MICROSCOPY (3.4 Å)