7ROC

Cryo-EM reconstruction of Sulfolobus monocaudavirus SMV1, symmetry 7

7ROC の概要

• エントリーDOI: 10.2210/pdb7roc/pdb
• EMDBエントリー: 24591
• 分子名称: major capsid protein (1 entity in total)
• 機能のキーワード: helical symmetry, archaeal virus, lemon-shaped virus, spindle-shaped virus, virus
• 由来する生物種: Sulfolobus monocaudavirus SMV1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16179.30

構造登録者

Wang, F.,Cvirkaite-Krupovic, V.,Krupovic, M.,Egelman, E.H. (登録日: 2021-07-30, 公開日: 2022-03-30, 最終更新日: 2024-06-05)

主引用文献

Wang, F.,Cvirkaite-Krupovic, V.,Vos, M.,Beltran, L.C.,Kreutzberger, M.A.B.,Winter, J.M.,Su, Z.,Liu, J.,Schouten, S.,Krupovic, M.,Egelman, E.H.
Spindle-shaped archaeal viruses evolved from rod-shaped ancestors to package a larger genome.
Cell, 185:1297-1307.e11, 2022

PubMed Abstract: Spindle- or lemon-shaped viruses infect archaea in diverse environments. Due to the highly pleomorphic nature of these virions, which can be found with cylindrical tails emanating from the spindle-shaped body, structural studies of these capsids have been challenging. We have determined the atomic structure of the capsid of Sulfolobus monocaudavirus 1, a virus that infects hosts living in nearly boiling acid. A highly hydrophobic protein, likely integrated into the host membrane before the virions assemble, forms 7 strands that slide past each other in both the tails and the spindle body. We observe the discrete steps that occur as the tail tubes expand, and these are due to highly conserved quasiequivalent interactions with neighboring subunits maintained despite significant diameter changes. Our results show how helical assemblies can vary their diameters, becoming nearly spherical to package a larger genome and suggest how all spindle-shaped viruses have evolved from archaeal rod-like viruses.

PubMed: 35325592
DOI: 10.1016/j.cell.2022.02.019

実験手法

ELECTRON MICROSCOPY (3.7 Å)