7RM6

Horse liver alcohol dehydrogenase in complex with NADH and N-cylcohexyl formamide

7RM6 の概要

• エントリーDOI: 10.2210/pdb7rm6/pdb
• 分子名称: Alcohol dehydrogenase E chain, ZINC ION, 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE, ... (5 entities in total)
• 機能のキーワード: hydride transfer, zinc metalloenzyme, oxidoreductase, oxidoreductase-inhibitor complex, oxidoreductase/inhibitor
• 由来する生物種: Equus caballus (Horse)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 81923.79

構造登録者

Zheng, C.,Boxer, S.G. (登録日: 2021-07-26, 公開日: 2022-04-27, 最終更新日: 2023-10-18)

主引用文献

Zheng, C.,Mao, Y.,Kozuch, J.,Atsango, A.O.,Ji, Z.,Markland, T.E.,Boxer, S.G.
A two-directional vibrational probe reveals different electric field orientations in solution and an enzyme active site.
Nat.Chem., 14:891-897, 2022

PubMed Abstract: The catalytic power of an electric field depends on its magnitude and orientation with respect to the reactive chemical species. Understanding and designing new catalysts for electrostatic catalysis thus requires methods to measure the electric field orientation and magnitude at the molecular scale. We demonstrate that electric field orientations can be extracted using a two-directional vibrational probe by exploiting the vibrational Stark effect of both the C=O and C-D stretches of a deuterated aldehyde. Combining spectroscopy with molecular dynamics and electronic structure partitioning methods, we demonstrate that, despite distinct polarities, solvents act similarly in their preference for electrostatically stabilizing large bond dipoles at the expense of destabilizing small ones. In contrast, we find that for an active-site aldehyde inhibitor of liver alcohol dehydrogenase, the electric field orientation deviates markedly from that found in solvents, which provides direct evidence for the fundamental difference between the electrostatic environment of solvents and that of a preorganized enzyme active site.

PubMed: 35513508
DOI: 10.1038/s41557-022-00937-w

実験手法

X-RAY DIFFRACTION (1.43 Å)