7RKC

Computationally designed tunable C2 symmetric tandem repeat homodimer, D_3_633

7RKC の概要

• エントリーDOI: 10.2210/pdb7rkc/pdb
• 分子名称: D_3_633, ACETATE ION (3 entities in total)
• 機能のキーワード: de novo, torroid, derroid, tandem repeat, homodimer, computationally designed, c2 symmetric, de novo protein
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 54551.20

構造登録者

Kennedy, M.A.,Stoddard, B.L.,Hicks, D.R.,Bera, A.K. (登録日: 2021-07-22, 公開日: 2022-05-18, 最終更新日: 2024-04-03)

主引用文献

Hicks, D.R.,Kennedy, M.A.,Thompson, K.A.,DeWitt, M.,Coventry, B.,Kang, A.,Bera, A.K.,Brunette, T.J.,Sankaran, B.,Stoddard, B.,Baker, D.
De novo design of protein homodimers containing tunable symmetric protein pockets.
Proc.Natl.Acad.Sci.USA, 119:e2113400119-e2113400119, 2022

PubMed Abstract: Function follows form in biology, and the binding of small molecules requires proteins with pockets that match the shape of the ligand. For design of binding to symmetric ligands, protein homo-oligomers with matching symmetry are advantageous as each protein subunit can make identical interactions with the ligand. Here, we describe a general approach to designing hyperstable C2 symmetric proteins with pockets of diverse size and shape. We first designed repeat proteins that sample a continuum of curvatures but have low helical rise, then docked these into C2 symmetric homodimers to generate an extensive range of C2 symmetric cavities. We used this approach to design thousands of C2 symmetric homodimers, and characterized 101 of them experimentally. Of these, the geometry of 31 were confirmed by small angle X-ray scattering and 2 were shown by crystallographic analyses to be in close agreement with the computational design models. These scaffolds provide a rich set of starting points for binding a wide range of C2 symmetric compounds.

PubMed: 35862457
DOI: 10.1073/pnas.2113400119

実験手法

X-RAY DIFFRACTION (2.35 Å)